Selective expression of sialyl-Lewis x and Lewis a epitopes, putative ligands for L-selectin, on peripheral lymph-node high endothelial venules
- PMID: 1281614
- PMCID: PMC1886755
Selective expression of sialyl-Lewis x and Lewis a epitopes, putative ligands for L-selectin, on peripheral lymph-node high endothelial venules
Abstract
High endothelial venules (HEV) lined by the high endothelium are the sites where leukocytes enter into the lymph nodes from the blood. Lymphocyte homing into lymph nodes is organ-selective, i.e., different molecules are involved in the lymphocyte homing to peripheral nodes compared with mucosa associated lymphoid tissue. The traffic into peripheral nodes is regulated by the expression of L-selectin on leukocytes and its ligand on HEVs. The ligand for L-selectin is suggested to be a 50, 90, or 105 kd glycoprotein, which is sulfated, fucosylated, and sialylated. The two other members of the selectin family (E- and P-selectin) recognize sialyl-Lewis x and -Lewis a (sLex and sLea, respectively) carbohydrate motifs, and there is preliminary data suggesting that this would also be the case for L-selectin. We have initiated a study to identify the expression of these sialylated structures on endothelial surfaces. We present data that show that HEVs in peripheral nodes, but not in the mucosa-associated lymphoid tissue, express large quantities of sLex and sLea identified by MAbs in immunohistology. Endothelium in capillaries or larger vessels in non-lymphoid tissues do not react with anti-sLex or -Lea mAbs. Only 1-2% of the lymphocytes in the peripheral blood express sLex and so far only the skin-homing lymphocytes are known to be sLex positive in larger quantities. We show that in many occasions the B cells in the peripheral lymph-node germinal centers are also sLex-, but not sLea-positive, and provide evidence of the restricted pattern of sLex and sLea expression on peripheral lymph-node HEVs. We propose that they are at least parts of the ligand for L-selectin.
Similar articles
-
Specific expression of a complex sialyl Lewis X antigen on high endothelial venules of human lymph nodes: possible candidate for L-selectin ligand.Biochem Biophys Res Commun. 1993 May 28;193(1):337-47. doi: 10.1006/bbrc.1993.1629. Biochem Biophys Res Commun. 1993. PMID: 7684905
-
L- and E-selectin can recognize the same naturally occurring ligands on high endothelial venules.J Immunol. 1993 Sep 15;151(6):3252-60. J Immunol. 1993. PMID: 7690798
-
Sulfated sialyl Lewis X, the putative L-selectin ligand, detected on endothelial cells of high endothelial venules by a distinct set of anti-sialyl Lewis X antibodies.Biochem Biophys Res Commun. 1997 Jan 23;230(3):546-51. doi: 10.1006/bbrc.1996.6012. Biochem Biophys Res Commun. 1997. PMID: 9015359
-
Integrins and L-selectin in lymphocyte-endothelium interactions and homing into gut-associated tissue.Behring Inst Mitt. 1993 Aug;(92):30-5. Behring Inst Mitt. 1993. PMID: 7504455 Review.
-
The selectin family of carbohydrate-binding proteins: structure and importance of carbohydrate ligands for cell adhesion.Bioessays. 1992 Dec;14(12):849-56. doi: 10.1002/bies.950141210. Bioessays. 1992. PMID: 1285423 Review.
Cited by
-
SELE gene as a characteristic prognostic biomarker of colorectal cancer.J Int Med Res. 2021 Apr;49(4):3000605211004386. doi: 10.1177/03000605211004386. J Int Med Res. 2021. PMID: 33845603 Free PMC article.
-
Biological roles of glycans.Glycobiology. 2017 Jan;27(1):3-49. doi: 10.1093/glycob/cww086. Epub 2016 Aug 24. Glycobiology. 2017. PMID: 27558841 Free PMC article. Review.
-
Essential domains of Anaplasma phagocytophilum invasins utilized to infect mammalian host cells.PLoS Pathog. 2015 Feb 6;11(2):e1004669. doi: 10.1371/journal.ppat.1004669. eCollection 2015 Feb. PLoS Pathog. 2015. PMID: 25658707 Free PMC article.
-
Diminished thrombogenic responses by deletion of the Podocalyxin Gene in mouse megakaryocytes.PLoS One. 2011;6(10):e26025. doi: 10.1371/journal.pone.0026025. Epub 2011 Oct 7. PLoS One. 2011. PMID: 22016802 Free PMC article.
-
Beyond mere markers: functions for CD34 family of sialomucins in hematopoiesis.Immunol Res. 2006;34(1):13-32. doi: 10.1385/IR:34:1:13. Immunol Res. 2006. PMID: 16720896 Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources