Impact of deletions within the Bam HI-L fragment of attenuated Marek's disease virus on vIL-8 expression and the newly identified transcript of open reading frame LORF4
- PMID: 12876454
- DOI: 10.1023/a:1024447230464
Impact of deletions within the Bam HI-L fragment of attenuated Marek's disease virus on vIL-8 expression and the newly identified transcript of open reading frame LORF4
Erratum in
- Virus Genes. 2005 Mar;30(2):297
Abstract
Marek's disease (MD) in chickens is caused by MD herpesvirus (MDV), which induces T cell lymphomas. The early pathogenesis of MDV infection is characterized by a primary infection in B lymphocytes followed by infection of activated T lymphocytes. It has been speculated that a MDV-encoded homologue of interleukin-8 (vIL-8) may be important to attract activated T lymphocytes to infected B lymphocytes. Recently, more virulent strains of MDV have emerged, named very virulent plus (vv+)MDV, that cause earlier and more prolonged cytolytic infections compared to less virulent strains. In this report, it was found that vIL-8 mRNA expression in vivo was increased in very virulent (vv) and vv+MDV strains compared to mild (m) and virulent (v) strains, and could not be detected in two attenuated MDV strains examined using very sensitive real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. In order to identify potential mechanisms for the increased vIL-8 mRNA expression in more virulent strains, and lack thereof in attenuated strains, the vIL-8 gene and putative promoter sequences upstream of the vIL-8 gene were compared from 10 different MDV strains, including attenuated derivatives. Only the JM-16 strain (both non-attenuated and attenuated) and attenuated 584A (584Ap80C) encoded a predicted vIL-8 gene sequence different from all other strains examined. Within the putative vIL-8 gene promoter sequence, there was little difference among the non-attenuated strains; however significant deletions were identified in the attenuated JM-16/p71, Md11 (R2/23), and 584Ap80C strains. Additionally, these deletions were located within a previously hypothetical open reading frame (ORF) named LORF4. Rapid amplification of cDNA ends identified a full-length transcript of LORF4 in the MDV-transformed lymphoblastoid cell line MSB-1, and deletions within this ORF caused truncated predicted proteins in 4 out of 6 attenuated MDV strains examined.
Similar articles
-
A Marek's disease virus vIL-8 deletion mutant has attenuated virulence and confers protection against challenge with a very virulent plus strain.Avian Dis. 2005 Jun;49(2):199-206. doi: 10.1637/7277-091004. Avian Dis. 2005. PMID: 16094823
-
Cloning of a very virulent plus, 686 strain of Marek's disease virus as a bacterial artificial chromosome.Avian Dis. 2013 Jun;57(2 Suppl):469-73. doi: 10.1637/10444-110412-ResNote.1. Avian Dis. 2013. PMID: 23901763
-
Marek's disease virus (MDV) encodes an interleukin-8 homolog (vIL-8): characterization of the vIL-8 protein and a vIL-8 deletion mutant MDV.J Virol. 2001 Jun;75(11):5159-73. doi: 10.1128/JVI.75.11.5159-5173.2001. J Virol. 2001. PMID: 11333897 Free PMC article.
-
Marek's disease vaccines: a solution for today but a worry for tomorrow?Vaccine. 2008 Jul 18;26 Suppl 3:C31-41. doi: 10.1016/j.vaccine.2008.04.009. Vaccine. 2008. PMID: 18773529 Review.
-
Latest Insights into Unique Open Reading Frames Encoded by Unique Long (UL) and Short (US) Regions of Marek's Disease Virus.Viruses. 2021 May 25;13(6):974. doi: 10.3390/v13060974. Viruses. 2021. PMID: 34070255 Free PMC article. Review.
Cited by
-
mRNA Splicing of UL44 and Secretion of Alphaherpesvirinae Glycoprotein C (gC) Is Conserved among the Mardiviruses.Viruses. 2024 May 15;16(5):782. doi: 10.3390/v16050782. Viruses. 2024. PMID: 38793663 Free PMC article.
-
The requirement of glycoprotein C (gC) for interindividual spread is a conserved function of gC for avian herpesviruses.Sci Rep. 2021 Apr 8;11(1):7753. doi: 10.1038/s41598-021-87400-x. Sci Rep. 2021. PMID: 33833367 Free PMC article.
-
Expression of the Conserved Herpesvirus Protein Kinase (CHPK) of Marek's Disease Alphaherpesvirus in the Skin Reveals a Mechanistic Importance for CHPK during Interindividual Spread in Chickens.J Virol. 2020 Feb 14;94(5):e01522-19. doi: 10.1128/JVI.01522-19. Print 2020 Feb 14. J Virol. 2020. PMID: 31801854 Free PMC article.
-
The Herpesviridae Conserved Multifunctional Infected-Cell Protein 27 (ICP27) Is Important but Not Required for Replication and Oncogenicity of Marek's Disease Alphaherpesvirus.J Virol. 2019 Feb 5;93(4):e01903-18. doi: 10.1128/JVI.01903-18. Print 2019 Feb 15. J Virol. 2019. PMID: 30518650 Free PMC article.
-
Marek's disease virus late protein expression in feather follicle epithelial cells as early as 8 days postinfection.Avian Dis. 2012 Dec;56(4):725-31. doi: 10.1637/10252-052212-Reg.1. Avian Dis. 2012. PMID: 23397845 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Miscellaneous