The mediator coactivator complex: functional and physical roles in transcriptional regulation
- PMID: 12917354
- DOI: 10.1242/jcs.00734
The mediator coactivator complex: functional and physical roles in transcriptional regulation
Abstract
In vivo, the DNA is packed into chromatin and transcription is dependent upon activators that recruit other factors to reverse the repressive effects of chromatin. The response to activators requires additional factors referred to as coactivators. One such coactivator, mediator, is a multi-subunit complex capable of responding to different activators. It plays an key role in activation, bridging DNA-bound activators, the general transcriptional machinery, especially RNA polymerase II, and the core promoter. Its subunits are necessary for a variety of positive and negative regulatory processes and serve as the direct targets of activators themselves. In vivo and in vitro studies support various roles for mediator in transcription initiation, while structural studies demonstrate that it engages in multiple interactions with RNA polymerase II, and adopts conformations that are activator specific.
Similar articles
-
Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo.Mol Cell Biol. 2005 Jul;25(13):5626-38. doi: 10.1128/MCB.25.13.5626-5638.2005. Mol Cell Biol. 2005. PMID: 15964818 Free PMC article.
-
A mediator required for activation of RNA polymerase II transcription in vitro.Nature. 1991 Apr 4;350(6317):436-8. doi: 10.1038/350436a0. Nature. 1991. PMID: 2011193
-
Functional characterization of core promoter elements: DPE-specific transcription requires the protein kinase CK2 and the PC4 coactivator.Mol Cell. 2005 May 13;18(4):471-81. doi: 10.1016/j.molcel.2005.04.005. Mol Cell. 2005. PMID: 15893730
-
Activation of RNA polymerase II transcription.Curr Opin Cell Biol. 1999 Jun;11(3):330-5. doi: 10.1016/S0955-0674(99)80045-3. Curr Opin Cell Biol. 1999. PMID: 10395559 Review.
-
Fluorescence resonance energy transfer as a method for dissecting in vivo mechanisms of transcriptional activation.Biochem Soc Symp. 2006;(73):217-24. Biochem Soc Symp. 2006. PMID: 16626301 Review.
Cited by
-
Biochemical Identification of a Nuclear Coactivator Protein Required for AtrR-Dependent Gene Regulation in Aspergillus fumigatus.mSphere. 2022 Dec 21;7(6):e0047622. doi: 10.1128/msphere.00476-22. Epub 2022 Nov 14. mSphere. 2022. PMID: 36374043 Free PMC article.
-
Mediator Med23 Regulates Adult Hippocampal Neurogenesis.Front Cell Dev Biol. 2020 Jul 29;8:699. doi: 10.3389/fcell.2020.00699. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 32850819 Free PMC article.
-
ErbB3-binding protein 1 (EBP1) represses HNF4α-mediated transcription and insulin secretion in pancreatic β-cells.J Biol Chem. 2019 Sep 20;294(38):13983-13994. doi: 10.1074/jbc.RA119.009558. Epub 2019 Jul 30. J Biol Chem. 2019. PMID: 31362984 Free PMC article.
-
Perturbing Enhancer Activity in Cancer Therapy.Cancers (Basel). 2019 May 7;11(5):634. doi: 10.3390/cancers11050634. Cancers (Basel). 2019. PMID: 31067678 Free PMC article. Review.
-
Regulation of the terminal maturation of iNKT cells by mediator complex subunit 23.Nat Commun. 2018 Sep 24;9(1):3875. doi: 10.1038/s41467-018-06372-1. Nat Commun. 2018. PMID: 30250136 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases