Comparison of coagulase-negative staphylococci by pulsed-field electrophoresis
- PMID: 1351017
- DOI: 10.1016/0378-1097(92)90501-e
Comparison of coagulase-negative staphylococci by pulsed-field electrophoresis
Abstract
Five pathogenic strains each of Staphylococcus epidermidis, S. haemolyticus, S. lugdunensis and S. schleiferi were analysed by conventional electrophoresis and field inversion gel electrophoresis. For these coagulase-negative staphylococci, the restriction endonuclease SmaI emerged as the most suitable enzyme for pulsed-field electrophoresis by providing an adequate number of clearly separated DNA fragments. Field inversion gel electrophoresis confirmed the differences among strains already discriminated by conventional electrophoresis, and furthermore, differentiated strains which had previously appeared identical. Among the species that were studied, S. epidermidis showed great genomic diversity with a few common bands. On the contrary, S. haemolyticus, S. lugdunensis and S. schleiferi showed less diversity. Although these minor variations may be epidemiologically significant, this question has to be investigated on a larger number of strains.
Similar articles
-
Staphylococcal cassette chromosome mec (SCC mec) in methicillin-resistant coagulase-negative staphylococci. A review and the experience in a tertiary-care setting.Epidemiol Infect. 2010 May;138(5):645-54. doi: 10.1017/S0950268809991361. Epub 2009 Dec 7. Epidemiol Infect. 2010. PMID: 19961645 Review.
-
Genomic variability of Staphylococcus aureus and the other coagulase-positive Staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis.Int J Syst Bacteriol. 1996 Jan;46(1):216-22. doi: 10.1099/00207713-46-1-216. Int J Syst Bacteriol. 1996. PMID: 8573498
-
Comparison of the SmaI-digested chromosomes of Staphylococcus epidermidis and the closely related species Staphylococcus capitis and Staphylococcus caprae.Int J Syst Bacteriol. 1994 Jul;44(3):404-9. doi: 10.1099/00207713-44-3-404. Int J Syst Bacteriol. 1994. PMID: 8068539
-
Pulsed-field gel electrophoresis of the genomic restriction fragments of coagulase-negative staphylococci.FEMS Microbiol Lett. 1994 Dec 1;124(2):131-9. doi: 10.1016/0378-1097(94)90240-2. FEMS Microbiol Lett. 1994. PMID: 7813882
-
Pulsed-field gel electrophoresis of genomic restriction fragments as a tool for the epidemiological analysis of Staphylococcus aureus and coagulase-negative staphylococci.FEMS Microbiol Lett. 1992 Aug 15;74(2-3):181-5. doi: 10.1016/0378-1097(92)90426-o. FEMS Microbiol Lett. 1992. PMID: 1326467
Cited by
-
Coagulase-negative staphylococci.Clin Microbiol Rev. 2014 Oct;27(4):870-926. doi: 10.1128/CMR.00109-13. Clin Microbiol Rev. 2014. PMID: 25278577 Free PMC article. Review.
-
From clinical microbiology to infection pathogenesis: how daring to be different works for Staphylococcus lugdunensis.Clin Microbiol Rev. 2008 Jan;21(1):111-33. doi: 10.1128/CMR.00036-07. Clin Microbiol Rev. 2008. PMID: 18202439 Free PMC article. Review.
-
Comparison of molecular typing methods for characterization of Staphylococcus epidermidis: proposal for clone definition.J Clin Microbiol. 2008 Jan;46(1):118-29. doi: 10.1128/JCM.01685-07. Epub 2007 Nov 7. J Clin Microbiol. 2008. PMID: 17989196 Free PMC article.
-
Inferring a population structure for Staphylococcus epidermidis from multilocus sequence typing data.J Bacteriol. 2007 Mar;189(6):2540-52. doi: 10.1128/JB.01484-06. Epub 2007 Jan 12. J Bacteriol. 2007. PMID: 17220222 Free PMC article.
-
Molecular characterization of methicillin-resistant Staphylococcus epidermidis clones: evidence of geographic dissemination.J Clin Microbiol. 2002 Feb;40(2):430-8. doi: 10.1128/JCM.40.2.430-438.2002. J Clin Microbiol. 2002. PMID: 11825953 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
