A proteomic analysis of arginine-methylated protein complexes
- PMID: 14534352
- DOI: 10.1074/mcp.M300088-MCP200
A proteomic analysis of arginine-methylated protein complexes
Abstract
Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA). This modification is catalyzed by type I and II protein-arginine methyltransferases (PRMT), respectively. The two major enzymes PRMT1 (type I) and PRMT5 (type II) preferentially methylate arginines located in RG-rich clusters. Arginine methylation is a common modification, but the reagents for detecting this modification have been lacking. Thus, fewer than 20 proteins have been identified in the last 40 years as containing dimethylated arginines. We have generated previously four arginine methyl-specific antibodies; ASYM24 and ASYM25 are specific for aDMA, whereas SYM10 and SYM11 recognize sDMA. All of these antibodies were generated by using peptides with aDMA or sDMA in the context of different RG-rich sequences. HeLa cell extracts were used to purify the protein complexes recognized by each of the four antibodies, and the proteins were identified by microcapillary reverse-phase liquid chromatography coupled on line with electrospray ionization tandem mass spectrometry. The analysis of two tandem mass spectra for each methyl-specific antibody resulted in the identification of over 200 new proteins that are putatively arginine-methylated. The major protein complexes that were purified include components required for pre-mRNA splicing, polyadenylation, transcription, signal transduction, and cytoskeleton and DNA repair. These findings provide a basis for the identification of the role of arginine methylation in many cellular processes.
Similar articles
-
GC-MS and LC-MS/MS pilot studies on the guanidine (NG)-dimethylation in native, asymmetrically and symmetrically NG-dimethylated arginine-vasopressin peptides and proteins in human red blood cells.J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Mar 15;1141:122024. doi: 10.1016/j.jchromb.2020.122024. Epub 2020 Feb 7. J Chromatogr B Analyt Technol Biomed Life Sci. 2020. PMID: 32062367
-
Cellular consequences of arginine methylation.Cell Mol Life Sci. 2019 Aug;76(15):2933-2956. doi: 10.1007/s00018-019-03140-2. Epub 2019 May 17. Cell Mol Life Sci. 2019. PMID: 31101937 Free PMC article. Review.
-
The story of protein arginine methylation: characterization, regulation, and function.Expert Rev Proteomics. 2017 Feb;14(2):157-170. doi: 10.1080/14789450.2017.1275573. Epub 2017 Jan 5. Expert Rev Proteomics. 2017. PMID: 28043171 Review.
-
Regulation of post-translational protein arginine methylation during HeLa cell cycle.Biochim Biophys Acta. 2010 Sep;1800(9):977-85. doi: 10.1016/j.bbagen.2010.06.004. Epub 2010 Jun 10. Biochim Biophys Acta. 2010. PMID: 20541591
-
Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein.RNA. 2001 Nov;7(11):1531-42. doi: 10.1017/s135583820101442x. RNA. 2001. PMID: 11720283 Free PMC article.
Cited by
-
Loss-of-function mutation in PRMT9 causes abnormal synapse development by dysregulation of RNA alternative splicing.Nat Commun. 2024 Apr 1;15(1):2809. doi: 10.1038/s41467-024-47107-9. Nat Commun. 2024. PMID: 38561334 Free PMC article.
-
Arginine methylation of SM-LIKE PROTEIN 4 antagonistically affects alternative splicing during Arabidopsis stress responses.Plant Cell. 2024 May 29;36(6):2219-2237. doi: 10.1093/plcell/koae051. Plant Cell. 2024. PMID: 38518124
-
Protein Arginine Methylation Patterns in Plasma Small Extracellular Vesicles Are Altered in Patients with Early-Stage Pancreatic Ductal Adenocarcinoma.Cancers (Basel). 2024 Feb 3;16(3):654. doi: 10.3390/cancers16030654. Cancers (Basel). 2024. PMID: 38339405 Free PMC article.
-
PRMT blockade induces defective DNA replication stress response and synergizes with PARP inhibition.Cell Rep Med. 2023 Dec 19;4(12):101326. doi: 10.1016/j.xcrm.2023.101326. Cell Rep Med. 2023. PMID: 38118413 Free PMC article.
-
Protein arginine methylation in viral infection and antiviral immunity.Int J Biol Sci. 2023 Oct 24;19(16):5292-5318. doi: 10.7150/ijbs.89498. eCollection 2023. Int J Biol Sci. 2023. PMID: 37928266 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources