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. 2004 Jan;36(1):37-41.
doi: 10.1093/abbs/36.1.37.

DNA vaccine of SARS-Cov S gene induces antibody response in mice

Ping Zhao  1 , Jin-Shan Ke, Zhao-Lin Qin, Hao Ren, Lan-Juan Zhao, Jian-Guo Yu, Jun Gao, Shi-Ying Zhu, Zhong-Tian Qi
Affiliations

DNA vaccine of SARS-Cov S gene induces antibody response in mice

Ping Zhao et al. Acta Biochim Biophys Sin (Shanghai). 2004 Jan.

Abstract

The spike (S) protein, a main surface antigen of SARS-coronavirus (SARS-CoV), is one of the most important antigen candidates for vaccine design. In the present study, three fragments of the truncated S protein were expressed in E.coli, and analyzed with pooled sera of convalescence phase of SARS patients. The full length S gene DNA vaccine was constructed and used to immunize BALB/c mice. The mouse serum IgG antibody against SARS-CoV was measured by ELISA with E. coli expressed truncated S protein or SARS-CoV lysate as diagnostic antigen. The results showed that all the three fragments of S protein expressed by E.coli was able to react with sera of SARS patients and the S gene DNA candidate vaccine could induce the production of specific IgG antibody against SARS-CoV efficiently in mice with seroconversion ratio of 75% after 3 times of immunization. These findings lay some foundations for further understanding the immunology of SARS-CoV and developing SARS vaccines.

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