The different roles of tryptophan transfer RNA in regulating trp operon expression in E. coli versus B. subtilis
- PMID: 15262409
- DOI: 10.1016/j.tig.2004.06.007
The different roles of tryptophan transfer RNA in regulating trp operon expression in E. coli versus B. subtilis
Abstract
Escherichia coli and Bacillus subtilis use different mechanisms of sensing and responding to tryptophan and uncharged tRNA(Trp) as regulatory signals. In E. coli, tryptophan activates a repressor that binds to the trp promoter- operator, inhibiting transcription initiation. In B. subtilis, tryptophan activates an RNA-binding protein, TRAP, which binds to the trp operon leader RNA, causing transcription termination. In E. coli uncharged tRNA(Trp) accumulation stalls the ribosome attempting translation of tandem Trp codons in the leader-peptide coding region of the operon. This stalling permits the formation of an RNA antiterminator structure, preventing transcription termination. In B. subtilis uncharged tRNA(Trp) accumulation activates transcription and translation of the at operon. AT protein inhibits tryptophan-activated TRAP, thereby preventing TRAP-mediated transcription termination. These differences might reflect the unique organizational features of the respective trp operons and their ancestry.
Similar articles
-
Tandem transcription and translation regulatory sensing of uncharged tryptophan tRNA.Science. 2003 Jul 11;301(5630):211-3. doi: 10.1126/science.1084902. Science. 2003. PMID: 12855807
-
Inhibition of the B. subtilis regulatory protein TRAP by the TRAP-inhibitory protein, AT.Science. 2001 Sep 14;293(5537):2057-9. doi: 10.1126/science.1062187. Science. 2001. PMID: 11557884
-
Effects of tryptophan starvation on levels of the trp RNA-binding attenuation protein (TRAP) and anti-TRAP regulatory protein and their influence on trp operon expression in Bacillus subtilis.J Bacteriol. 2005 Mar;187(6):1884-91. doi: 10.1128/JB.187.6.1884-1891.2005. J Bacteriol. 2005. PMID: 15743934 Free PMC article.
-
Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis.Annu Rev Genet. 2005;39:47-68. doi: 10.1146/annurev.genet.39.073003.093745. Annu Rev Genet. 2005. PMID: 16285852 Review.
-
Some novel transcription attenuation mechanisms used by bacteria.Biochimie. 1996;78(11-12):1017-24. doi: 10.1016/s0300-9084(97)86725-9. Biochimie. 1996. PMID: 9150880 Review.
Cited by
-
Static Magnetic Field Inhibits Growth of Escherichia coli Colonies via Restriction of Carbon Source Utilization.Cells. 2022 Feb 27;11(5):827. doi: 10.3390/cells11050827. Cells. 2022. PMID: 35269449 Free PMC article.
-
Evolution of new enzymes by gene duplication and divergence.FEBS J. 2020 Apr;287(7):1262-1283. doi: 10.1111/febs.15299. FEBS J. 2020. PMID: 32250558 Free PMC article. Review.
-
Conserved genomic neighborhood is a strong but no perfect indicator for a direct interaction of microbial gene products.BMC Bioinformatics. 2020 Jan 3;21(1):5. doi: 10.1186/s12859-019-3200-z. BMC Bioinformatics. 2020. PMID: 31900122 Free PMC article.
-
Repetitive Short-Term Stimuli Imposed in Poor Mixing Zones Induce Long-Term Adaptation of E. coli Cultures in Large-Scale Bioreactors: Experimental Evidence and Mathematical Model.Front Microbiol. 2017 Jun 28;8:1195. doi: 10.3389/fmicb.2017.01195. eCollection 2017. Front Microbiol. 2017. PMID: 28702020 Free PMC article.
-
Specialized metabolites from the microbiome in health and disease.Cell Metab. 2014 Nov 4;20(5):719-730. doi: 10.1016/j.cmet.2014.10.016. Epub 2014 Nov 4. Cell Metab. 2014. PMID: 25440054 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
