Subcellullar localization of tumor-associated antigen 3H11Ag
- PMID: 15474516
- DOI: 10.1016/j.bbrc.2004.09.133
Subcellullar localization of tumor-associated antigen 3H11Ag
Abstract
3H11Ag, a tumor-associated antigen defined by the monoclonal antibody 3H11 that specifically recognizes cancer cells in various tumor tissues, was successfully cloned recently, but its function is unknown. To explore the potential roles it plays in tumors, we analyzed its subcellular localization in the present study. By expressing 3H11Ag fused with fluorescent protein in COS-7 cells, we found that 3H11Ag localizes to both cytoplasm and nucleus, which was confirmed by subcellular fractionation. And sequentially extracting the nuclei of COS-7 cells transfected with 3H11Ag showed that it is a DNA- and nuclear matrix-associated protein. Moreover, by expressing a series of red fluorescent protein-tagged truncated forms of 3H11Ag, it was demonstrated that the 150 amino acid residues at its C-terminal are fully responsible for the subcellular localization. In addition, the results of the computational analysis of 3H11Ag were in accordance with those of the experimental analysis. All these data would be helpful to elucidate the functions of 3H11Ag.
Similar articles
-
An anti-apoptotic protein, Hax-1, inhibits the HIV-1 rev function by altering its sub-cellular localization.J Cell Physiol. 2008 Jan;214(1):14-9. doi: 10.1002/jcp.21305. J Cell Physiol. 2008. PMID: 17929250
-
Localization of human T-cell lymphotropic virus type II Tax protein is dependent upon a nuclear localization determinant in the N-terminal region.Gene. 2006 Jan 3;365:119-24. doi: 10.1016/j.gene.2005.09.043. Epub 2005 Dec 6. Gene. 2006. PMID: 16337343
-
Spindlin1, a novel nuclear protein with a role in the transformation of NIH3T3 cells.Biochem Biophys Res Commun. 2005 Sep 23;335(2):343-50. doi: 10.1016/j.bbrc.2005.07.087. Biochem Biophys Res Commun. 2005. PMID: 16098913
-
[Eukaryotic expression and intracellular distribution of cancer and embryo expression protein 65].Ai Zheng. 2005 May;24(5):573-6. Ai Zheng. 2005. PMID: 15890100 Chinese.
-
Nuclear and plasma membrane localization of SH3BP4 in retinal pigment epithelial cells.Mol Vis. 2004 Dec 13;10:933-42. Mol Vis. 2004. PMID: 15616480
Cited by
-
Cancer and embryo expression protein 65 promotes cancer cell growth and metastasis.Oncol Lett. 2015 Apr;9(4):1772-1778. doi: 10.3892/ol.2015.2958. Epub 2015 Feb 12. Oncol Lett. 2015. PMID: 25789040 Free PMC article.
-
CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content.J Cell Biol. 2010 Sep 6;190(5):927-40. doi: 10.1083/jcb.201006105. J Cell Biol. 2010. PMID: 20819941 Free PMC article.
-
RPGR-containing protein complexes in syndromic and non-syndromic retinal degeneration due to ciliary dysfunction.J Genet. 2009 Dec;88(4):399-407. doi: 10.1007/s12041-009-0061-7. J Genet. 2009. PMID: 20090203 Free PMC article. Review.
-
Mutations in the human naked cuticle homolog NKD1 found in colorectal cancer alter Wnt/Dvl/beta-catenin signaling.PLoS One. 2009 Nov 24;4(11):e7982. doi: 10.1371/journal.pone.0007982. PLoS One. 2009. PMID: 19956716 Free PMC article.
-
In-frame deletion in a novel centrosomal/ciliary protein CEP290/NPHP6 perturbs its interaction with RPGR and results in early-onset retinal degeneration in the rd16 mouse.Hum Mol Genet. 2006 Jun 1;15(11):1847-57. doi: 10.1093/hmg/ddl107. Epub 2006 Apr 21. Hum Mol Genet. 2006. PMID: 16632484 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
