From candelas to photoisomerizations in the mouse eye by rhodopsin bleaching in situ and the light-rearing dependence of the major components of the mouse ERG
- PMID: 15535992
- DOI: 10.1016/j.visres.2004.09.019
From candelas to photoisomerizations in the mouse eye by rhodopsin bleaching in situ and the light-rearing dependence of the major components of the mouse ERG
Abstract
To quantify the rate at which light in a ganzfeld produces photoisomerizations in mouse rods in situ, we measured the rate of rhodopsin bleaching in eyes of recently euthanized mice with fully dilated pupils. The amount of rhodopsin declined as a first-order (exponential) function of the duration of the exposure at the luminance of 920 scot cd m(-2): the rate constants of bleaching were 8.3 x 10(-6) and 2.8 x 10(-5) s(-1) (scot cd(-1)m2)(-1) for C57B1/6 and 129P3/J mice, respectively. When the approximately 3-fold difference in effective areas of the pupils of the mice are taken into consideration, the bleaching rates for both strains become essentially the same, 2.6 x 10(-6) fraction rhodopsin (scot Td s)(-1). Assuming 7 x 10(7) rhodopsin molecules per rod, this bleaching rate yields the result that a flash of 1 scot Td s produces 181 photoisomerizations per rod, a value close to that derived from analysis of the collecting area of the rod for axially propagating light. We measured the electroretinograms of mice of the two strains reared under controlled illumination conditions (2 and 100 lux), and compared their properties, using the calibrations to determine the absolute sensitivities of the b-wave and a-waves. The intensity that produces a half-saturating rod b-wave response is 0.3-0.6 photoisomerizations rod(-1), and the amplification constant of the rod a-wave is 5-6 s(-2) photoisomerization(-1), with little dependence on the strain.
Similar articles
-
Recovery of rod photoresponses in ABCR-deficient mice.Invest Ophthalmol Vis Sci. 2008 Jun;49(6):2743-55. doi: 10.1167/iovs.07-1499. Epub 2008 Feb 8. Invest Ophthalmol Vis Sci. 2008. PMID: 18263807 Free PMC article.
-
The time course of light adaptation in vertebrate retinal rods.Adv Exp Med Biol. 2002;514:37-60. doi: 10.1007/978-1-4615-0121-3_3. Adv Exp Med Biol. 2002. PMID: 12596914 Review.
-
Light adaptation and dark adaptation of human rod photoreceptors measured from the a-wave of the electroretinogram.J Physiol. 1999 Jul 15;518 ( Pt 2)(Pt 2):479-96. doi: 10.1111/j.1469-7793.1999.0479p.x. J Physiol. 1999. PMID: 10381594 Free PMC article. Clinical Trial.
-
Effect of eye closures and openings on photostasis in albino rats.Invest Ophthalmol Vis Sci. 1998 Mar;39(3):603-9. Invest Ophthalmol Vis Sci. 1998. PMID: 9501872
-
Assessing abnormal rod photoreceptor activity with the a-wave of the electroretinogram: applications and methods.Doc Ophthalmol. 1996-1997;92(4):253-67. doi: 10.1007/BF02584080. Doc Ophthalmol. 1996. PMID: 9476593 Review.
Cited by
-
A metabotropic glutamate receptor agonist enhances visual signal fidelity in a mouse model of retinitis pigmentosa.bioRxiv [Preprint]. 2024 Apr 30:2024.04.30.591881. doi: 10.1101/2024.04.30.591881. bioRxiv. 2024. PMID: 38746092 Free PMC article. Preprint.
-
Super-resolution mapping in rod photoreceptors identifies rhodopsin trafficking through the inner segment plasma membrane as an essential subcellular pathway.PLoS Biol. 2024 Jan 8;22(1):e3002467. doi: 10.1371/journal.pbio.3002467. eCollection 2024 Jan. PLoS Biol. 2024. PMID: 38190419 Free PMC article.
-
Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography.Life Sci Alliance. 2024 Jan 5;7(3):e202302409. doi: 10.26508/lsa.202302409. Print 2024 Mar. Life Sci Alliance. 2024. PMID: 38182160 Free PMC article.
-
Layers of inhibitory networks shape receptive field properties of AII amacrine cells.Cell Rep. 2023 Nov 28;42(11):113390. doi: 10.1016/j.celrep.2023.113390. Epub 2023 Nov 5. Cell Rep. 2023. PMID: 37930888 Free PMC article.
-
Gene Therapy in Hereditary Retinal Dystrophies: The Usefulness of Diagnostic Tools in Candidate Patient Selections.Int J Mol Sci. 2023 Sep 6;24(18):13756. doi: 10.3390/ijms241813756. Int J Mol Sci. 2023. PMID: 37762059 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources