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. 2004 Dec;70(12):7558-60.
doi: 10.1128/AEM.70.12.7558-7560.2004.

Resistance of solid-phase U(VI) to microbial reduction during in situ bioremediation of uranium-contaminated groundwater

Irene Ortiz-Bernad  1 , Robert T Anderson, Helen A Vrionis, Derek R Lovley
Affiliations

Resistance of solid-phase U(VI) to microbial reduction during in situ bioremediation of uranium-contaminated groundwater

Irene Ortiz-Bernad et al. Appl Environ Microbiol. 2004 Dec.

Abstract

Speciation of solid-phase uranium in uranium-contaminated subsurface sediments undergoing uranium bioremediation demonstrated that although microbial reduction of soluble U(VI) readily immobilized uranium as U(IV), a substantial portion of the U(VI) in the aquifer was strongly associated with the sediments and was not microbially reducible. These results have important implications for in situ uranium bioremediation strategies.

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Figures

FIG. 1.
FIG. 1.
Percentages of U(VI) out of the total U of sediment cores collected at three different depths in a uranium-bioremediation site located in Rifle, Colo. The dashed line indicates the position of the acetate injection gallery. P11, upgradient sediment core; P12 to P15, downgradient cores from the point of acetate injection into the subsurface. The results are the means of results of five replicates, and the standard deviations were below 10% of the means.
FIG. 2.
FIG. 2.
Sediment and soluble uranium, sulfate, and ratio of Fe(II) to total acid-extractable iron (Fet) in contaminated aquifer sediments (115 g) with uranium-contaminated groundwater (30 ml) incubated with (B) or without (A) added acetate (2 mM). The results are the means of results of triplicate sediment incubations.
FIG. 3.
FIG. 3.
Sediment and soluble uranium, sulfate, and ratio of Fe(II) to total acid-extractable iron (Fet) in contaminated aquifer sediments (115 g) with uranium-contaminated groundwater (30 ml) amended with an additional 20 μM U(VI) and incubated with (B) or without (A) added acetate (5 mM). The results are the means of results of quintuplet extractions made on duplicate sediment incubations.
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References

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