Regulation of miRNA expression during neural cell specification
- PMID: 15845075
- DOI: 10.1111/j.1460-9568.2005.03978.x
Regulation of miRNA expression during neural cell specification
Abstract
MicroRNA (miRNA) are a newly recognized class of small, noncoding RNA molecules that participate in the developmental control of gene expression. We have studied the regulation of a set of highly expressed neural miRNA during mouse brain development. Temporal control is a characteristic of miRNA regulation in C. elegans and Drosophila, and is also prominent in the embryonic brain. We observed significant differences in the onset and magnitude of induction for individual miRNAs. Comparing expression in cultures of embryonic neurons and astrocytes we found marked lineage specificity for each of the miRNA in our study. Two of the most highly expressed miRNA in adult brain were preferentially expressed in neurons (mir-124, mir-128). In contrast, mir-23, a miRNA previously implicated in neural specification, was restricted to astrocytes. mir-26 and mir-29 were more strongly expressed in astrocytes than neurons, others were more evenly distributed (mir-9, mir-125). Lineage specificity was further explored using reporter constructs for two miRNA of particular interest (mir-125 and mir-128). miRNA-mediated suppression of both reporters was observed after transfection of the reporters into neurons but not astrocytes. miRNA were strongly induced during neural differentiation of embryonic stem cells, suggesting the validity of the stem cell model for studying miRNA regulation in neural development.
Similar articles
-
Marked change in microRNA expression during neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonal carcinoma P19 cells.Biochem Biophys Res Commun. 2007 Oct 19;362(2):360-7. doi: 10.1016/j.bbrc.2007.07.189. Epub 2007 Aug 13. Biochem Biophys Res Commun. 2007. PMID: 17716626
-
MicroRNAs regulate synthesis of the neurotransmitter substance P in human mesenchymal stem cell-derived neuronal cells.Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15484-9. doi: 10.1073/pnas.0703037104. Epub 2007 Sep 13. Proc Natl Acad Sci U S A. 2007. PMID: 17855557 Free PMC article.
-
Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles.Mol Hum Reprod. 2008 Sep;14(9):521-9. doi: 10.1093/molehr/gan044. Epub 2008 Aug 12. Mol Hum Reprod. 2008. PMID: 18697907
-
MicroRNAs regulation modulated self-renewal and lineage differentiation of stem cells.Cell Transplant. 2009;18(9):1039-45. doi: 10.3727/096368909X471224. Epub 2009 Apr 29. Cell Transplant. 2009. PMID: 19523330 Review.
-
MicroRNAs in muscle differentiation: lessons from Drosophila and beyond.Curr Opin Genet Dev. 2006 Oct;16(5):533-9. doi: 10.1016/j.gde.2006.08.010. Epub 2006 Aug 17. Curr Opin Genet Dev. 2006. PMID: 16919443 Review.
Cited by
-
Can miRNA Expression Levels Predict Treatment Resistance to Serotonin Reuptake Inhibitors in Patients with Obsessive-Compulsive Disorder?Psychiatry Clin Psychopharmacol. 2022 Jun 1;32(2):98-106. doi: 10.5152/pcp.2022.22391. eCollection 2022 Jun. Psychiatry Clin Psychopharmacol. 2022. PMID: 38764867 Free PMC article.
-
Stem Cell-Derived Exosomal MicroRNAs as Novel Potential Approach for Multiple Sclerosis Treatment.Cell Mol Neurobiol. 2024 May 7;44(1):44. doi: 10.1007/s10571-024-01478-1. Cell Mol Neurobiol. 2024. PMID: 38713302 Free PMC article. Review.
-
MiRNAs from the Dlk1-Dio3 locus and miR-224/452 cluster contribute to glioblastoma tumor heterogeneity.Sci Rep. 2024 Apr 13;14(1):8570. doi: 10.1038/s41598-024-58870-6. Sci Rep. 2024. PMID: 38609422 Free PMC article.
-
MiR-128-3p - a gray eminence of the human central nervous system.Mol Ther Nucleic Acids. 2024 Feb 6;35(1):102141. doi: 10.1016/j.omtn.2024.102141. eCollection 2024 Mar 12. Mol Ther Nucleic Acids. 2024. PMID: 38419943 Free PMC article. Review.
-
Simple and Highly Specific Targeting of Resident Microglia with Adeno-Associated Virus.bioRxiv [Preprint]. 2023 Dec 13:2023.12.12.571321. doi: 10.1101/2023.12.12.571321. bioRxiv. 2023. PMID: 38168285 Free PMC article. Preprint.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases