A 'loop recapture' mechanism for ACF-dependent nucleosome remodeling
- PMID: 16025127
- DOI: 10.1038/nsmb966
A 'loop recapture' mechanism for ACF-dependent nucleosome remodeling
Abstract
The ATPase ISWI is the molecular motor of several nucleosome remodeling complexes including ACF. We analyzed the ACF-nucleosome interactions and determined the characteristics of ACF-dependent nucleosome remodeling. In contrast to ISWI, ACF interacts symmetrically with DNA entry sites of the nucleosome. Two-color fluorescence cross-correlation spectroscopy measurements show that ACF can bind four DNA duplexes simultaneously in a complex that contains two Acf1 and ISWI molecules. Using bead-bound nucleosomal substrates, nucleosome movement by mechanisms involving DNA twisting was excluded. Furthermore, an ACF-dependent local detachment of DNA from the nucleosome was demonstrated in a novel assay based on the preferred intercalation of ethidium bromide to free DNA. The findings suggest a loop recapture mechanism in which ACF introduces a DNA loop at the nucleosomal entry site that propagates over the histone octamer surface and leads to nucleosome repositioning.
Comment in
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Nucleosome remodelers on track.Nat Struct Mol Biol. 2005 Sep;12(9):732-3. doi: 10.1038/nsmb0905-732. Nat Struct Mol Biol. 2005. PMID: 16142222 No abstract available.
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