A quantitative matrigel assay for assessing repopulating capacity of prostate stem cells
- PMID: 16286093
- DOI: 10.1016/j.bbrc.2005.10.055
A quantitative matrigel assay for assessing repopulating capacity of prostate stem cells
Abstract
Homeostasis of prostate tissue is maintained by stem cells, although such cells have not been well characterized. Here, we report establishment of such a method using matrigel. Matrigel containing a single-cell suspension from adult prostatic cells was subcutaneously grafted into the flank of nude mice. Prostatic duct-like structures derived from donor tissue were observed in the gel 2 weeks after transplantation. Luminal and basal cells observed in the gel expressed several markers characteristic of prostatic and/or epithelial cells. When a mixture with both EGFP-positive and negative prostate cells was transplanted, prostatic ducts consisted of either EGFP-positive or negative cells and chimeric patterns were rarely observed, suggesting that ducts were reconstituted from a single cell. Stem cell number and function were also evaluated by competition with control cells. Overall this method revealed that cells localized in the proximal portion in prostate ducts had higher reconstitution capacity than those in the distal portion. We conclude that prostate stem/progenitor cells exist and that our method is applicable to analysis of prostate stem cells, epithelial mesenchyme interactions, and prostate cancer stem cells.
Similar articles
-
Epithelial stem cells of the prostate and their role in cancer progression.Cold Spring Harb Symp Quant Biol. 2008;73:491-502. doi: 10.1101/sqb.2008.73.012. Epub 2008 Nov 6. Cold Spring Harb Symp Quant Biol. 2008. PMID: 19022743 Review.
-
Micropatterns of Matrigel for three-dimensional epithelial cultures.Biomaterials. 2007 Sep;28(27):4006-16. doi: 10.1016/j.biomaterials.2007.05.021. Epub 2007 Jun 15. Biomaterials. 2007. PMID: 17574663
-
Peripheral nerve regeneration using a three dimensionally cultured schwann cell conduit.J Craniofac Surg. 2007 May;18(3):475-88. doi: 10.1097/01.scs.0000249362.41170.f3. J Craniofac Surg. 2007. PMID: 17538306
-
Proximal prostatic stem cells are programmed to regenerate a proximal-distal ductal axis.Stem Cells. 2006 Aug;24(8):1859-68. doi: 10.1634/stemcells.2005-0585. Epub 2006 Apr 27. Stem Cells. 2006. PMID: 16644920
-
The potential for the use of nanofeaturing in medical devices.Expert Rev Med Devices. 2005 May;2(3):293-301. doi: 10.1586/17434440.2.3.293. Expert Rev Med Devices. 2005. PMID: 16288593 Review.
Cited by
-
Re-epithelialization of the prostatic urethra after two-micron laser resection of the prostate.Asian J Androl. 2016 Jan-Feb;18(1):151-3. doi: 10.4103/1008-682X.154993. Asian J Androl. 2016. PMID: 26178394 Free PMC article. No abstract available.
-
Prostate stem cells in the development of benign prostate hyperplasia and prostate cancer: emerging role and concepts.Biomed Res Int. 2013;2013:107954. doi: 10.1155/2013/107954. Epub 2013 Jul 8. Biomed Res Int. 2013. PMID: 23936768 Free PMC article. Review.
-
Searching for prostate cancer stem cells: markers and methods.Stem Cell Rev Rep. 2013 Oct;9(5):721-30. doi: 10.1007/s12015-013-9453-4. Stem Cell Rev Rep. 2013. PMID: 23775699 Review.
-
SATB1 defines the developmental context for gene silencing by Xist in lymphoma and embryonic cells.Dev Cell. 2009 Apr;16(4):507-16. doi: 10.1016/j.devcel.2009.03.006. Dev Cell. 2009. PMID: 19386260 Free PMC article.
-
Stem cells in prostate cancer initiation and progression.J Clin Invest. 2007 Aug;117(8):2044-50. doi: 10.1172/JCI32810. J Clin Invest. 2007. PMID: 17671638 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
