Stability of Marek's disease virus 132-bp repeats during serial in vitro passages
- PMID: 16489507
- DOI: 10.1007/s00705-006-0724-0
Stability of Marek's disease virus 132-bp repeats during serial in vitro passages
Abstract
The Marek's disease virus (MDV) genome contains 2 sets of 132-bp tandem repeat sequences. An increase in 132-bp repeat units has been associated with attenuation of oncogenicity during in vitro passage. By cloning entire genomes, we demonstrated that the copy number of 132-bp repeats can differ within an individual MDV genome. The stability of the 132-bp repeats during cell passage depended on the initial copy number. When both sets of repeats contained 2 copies, the copy number remained stable, while if even 1 set of repeats contained 6 copies, repeat expansion occurred relatively quickly. This expansion did not affect the in vitro growth curve. However, when MDV clones with low and high copy numbers were passed together, genomes with expanded repeats rapidly predominated, mimicking the behavior of naturally-occurring MDV. These results suggest that the preponderance of high-copy repeats after passage reflects intracellular copy number within individual infected cells rather than an influence on the spread of the virus.
Similar articles
-
Copy number of tandem direct repeats within the inverted repeats of Marek's disease virus DNA.Biken J. 1986 Dec;29(3-4):83-9. Biken J. 1986. PMID: 2441693
-
Direct evidence of host genome acquisition by the alphaherpesvirus Marek's disease virus.Arch Virol. 2006 Mar;151(3):537-49. doi: 10.1007/s00705-005-0633-7. Epub 2005 Sep 9. Arch Virol. 2006. PMID: 16155725
-
Genome analysis of Marek's disease virus strain CVI-988: effect of cell culture passage on the inverted repeat regions.Avian Dis. 1999 Apr-Jun;43(2):182-8. Avian Dis. 1999. PMID: 10396630
-
The dynamic herpesvirus DNA genome: the case of MDV-1 and HSV-1.Acta Virol. 1999 Apr-Jun;43(2-3):81-9. Acta Virol. 1999. PMID: 10696425 Review.
-
Vaccinal control of Marek's disease: current challenges, and future strategies to maximize protection.Vet Immunol Immunopathol. 2006 Jul 15;112(1-2):78-86. doi: 10.1016/j.vetimm.2006.03.014. Epub 2006 May 8. Vet Immunol Immunopathol. 2006. PMID: 16682084 Review.
Cited by
-
Real-time PCR for differential quantification of CVI988 vaccine virus and virulent strains of Marek's disease virus.J Virol Methods. 2016 Jul;233:23-36. doi: 10.1016/j.jviromet.2016.03.002. Epub 2016 Mar 10. J Virol Methods. 2016. PMID: 26973285 Free PMC article.
-
Dynamic equilibrium of Marek's disease genomes during in vitro serial passage.Virus Genes. 2012 Dec;45(3):526-36. doi: 10.1007/s11262-012-0792-z. Epub 2012 Aug 26. Virus Genes. 2012. PMID: 22923089
-
Comparative full genome analysis of four infectious laryngotracheitis virus (Gallid herpesvirus-1) virulent isolates from the United States.Virus Genes. 2012 Apr;44(2):273-85. doi: 10.1007/s11262-011-0696-3. Epub 2011 Dec 16. Virus Genes. 2012. PMID: 22173980
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources