Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells
- PMID: 16699504
- DOI: 10.1038/ncb1413
Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells
Abstract
Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5). This SET-PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster. Blimp1-Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1-Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells. Subsequently, Dhx38, a putative target of the Blimp1-Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.
Similar articles
-
Expression of BLIMP1/PRMT5 and concurrent histone H2A/H4 arginine 3 dimethylation in fetal germ cells, CIS/IGCNU and germ cell tumors.BMC Dev Biol. 2008 Nov 7;8:106. doi: 10.1186/1471-213X-8-106. BMC Dev Biol. 2008. PMID: 18992153 Free PMC article.
-
OGG1 regulates the level of symmetric dimethylation of histone H4 arginine-3 by interacting with PRMT5.Mol Cell Probes. 2018 Apr;38:19-24. doi: 10.1016/j.mcp.2018.01.002. Epub 2018 Feb 2. Mol Cell Probes. 2018. PMID: 29409673
-
The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice.EMBO J. 2015 Mar 12;34(6):748-58. doi: 10.15252/embj.201489319. Epub 2014 Dec 17. EMBO J. 2015. PMID: 25519955 Free PMC article.
-
Germ cell specification in mice.Science. 2007 Apr 20;316(5823):394-6. doi: 10.1126/science.1137545. Science. 2007. PMID: 17446386 Review.
-
The PRMT5 arginine methyltransferase: many roles in development, cancer and beyond.Cell Mol Life Sci. 2015 Jun;72(11):2041-59. doi: 10.1007/s00018-015-1847-9. Epub 2015 Feb 7. Cell Mol Life Sci. 2015. PMID: 25662273 Free PMC article. Review.
Cited by
-
An epigenetic timer regulates the transition from cell division to cell expansion during Arabidopsis petal organogenesis.PLoS Genet. 2024 Mar 5;20(3):e1011203. doi: 10.1371/journal.pgen.1011203. eCollection 2024 Mar. PLoS Genet. 2024. PMID: 38442104 Free PMC article.
-
Genetic screen identified PRMT5 as a neuroprotection target against cerebral ischemia.Elife. 2024 Feb 19;12:RP89754. doi: 10.7554/eLife.89754. Elife. 2024. PMID: 38372724 Free PMC article.
-
Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?Hortic Res. 2023 Aug 29;10(10):uhad174. doi: 10.1093/hr/uhad174. eCollection 2023 Oct. Hortic Res. 2023. PMID: 37841501 Free PMC article.
-
Nuclear PRMT5 is a biomarker of sensitivity to tamoxifen in ERα+ breast cancer.EMBO Mol Med. 2023 Aug 7;15(8):e17248. doi: 10.15252/emmm.202217248. Epub 2023 Jul 17. EMBO Mol Med. 2023. PMID: 37458145 Free PMC article.
-
PRMT5 links lipid metabolism to contractile function of skeletal muscles.EMBO Rep. 2023 Aug 3;24(8):e57306. doi: 10.15252/embr.202357306. Epub 2023 Jun 19. EMBO Rep. 2023. PMID: 37334900 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials