INCENP and Aurora B promote meiotic sister chromatid cohesion through localization of the Shugoshin MEI-S332 in Drosophila
- PMID: 16824953
- PMCID: PMC7115953
- DOI: 10.1016/j.devcel.2006.04.021
INCENP and Aurora B promote meiotic sister chromatid cohesion through localization of the Shugoshin MEI-S332 in Drosophila
Abstract
The chromosomal passenger complex protein INCENP is required in mitosis for chromosome condensation, spindle attachment and function, and cytokinesis. Here, we show that INCENP has an essential function in the specialized behavior of centromeres in meiosis. Mutations affecting Drosophila incenp profoundly affect chromosome segregation in both meiosis I and II, due, at least in part, to premature sister chromatid separation in meiosis I. INCENP binds to the cohesion protector protein MEI-S332, which is also an excellent in vitro substrate for Aurora B kinase. A MEI-S332 mutant that is only poorly phosphorylated by Aurora B is defective in localization to centromeres. These results implicate the chromosomal passenger complex in directly regulating MEI-S332 localization and, therefore, the control of sister chromatid cohesion in meiosis.
Figures
![Figure 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f001.gif)
![Figure 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f002.gif)
![Figure 3](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f003.gif)
![Figure 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f004.gif)
![Figure 5](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f005.gif)
![Figure 6](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f006.gif)
![Figure 7](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/7115953/bin/EMS90682-f007.gif)
Similar articles
-
Regulation of centromere localization of the Drosophila Shugoshin MEI-S332 and sister-chromatid cohesion in meiosis.G3 (Bethesda). 2014 Jul 31;4(10):1849-58. doi: 10.1534/g3.114.012823. G3 (Bethesda). 2014. PMID: 25081981 Free PMC article.
-
Roles and regulation of the Drosophila centromere cohesion protein MEI-S332 family.Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):543-52. doi: 10.1098/rstb.2005.1619. Philos Trans R Soc Lond B Biol Sci. 2005. PMID: 15915585 Free PMC article.
-
Maintenance of sister-chromatid cohesion at the centromere by the Drosophila MEI-S332 protein.Genes Dev. 1998 Dec 15;12(24):3843-56. doi: 10.1101/gad.12.24.3843. Genes Dev. 1998. PMID: 9869638 Free PMC article.
-
Shugoshin protects cohesin complexes at centromeres.Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):515-21, discussion 521. doi: 10.1098/rstb.2004.1607. Philos Trans R Soc Lond B Biol Sci. 2005. PMID: 15897177 Free PMC article. Review.
-
Chromosomal passengers: conducting cell division.Nat Rev Mol Cell Biol. 2007 Oct;8(10):798-812. doi: 10.1038/nrm2257. Nat Rev Mol Cell Biol. 2007. PMID: 17848966 Review.
Cited by
-
Weighted Gene Co-Expression Network Analysis Based on Stimulation by Lipopolysaccharides and Polyinosinic:polycytidylic Acid Provides a Core Set of Genes for Understanding Hemolymph Immune Response Mechanisms of Amphioctopus fangsiao.Animals (Basel). 2023 Dec 25;14(1):80. doi: 10.3390/ani14010080. Animals (Basel). 2023. PMID: 38200810 Free PMC article.
-
Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.Commun Biol. 2023 Aug 15;6(1):850. doi: 10.1038/s42003-023-05224-z. Commun Biol. 2023. PMID: 37582841 Free PMC article.
-
Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast.Mol Biol Cell. 2022 Aug 1;33(9):ar85. doi: 10.1091/mbc.E21-11-0590. Epub 2022 Jun 15. Mol Biol Cell. 2022. PMID: 35704464 Free PMC article.
-
The phospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin.PLoS Genet. 2022 Jun 6;18(6):e1009995. doi: 10.1371/journal.pgen.1009995. eCollection 2022 Jun. PLoS Genet. 2022. PMID: 35666772 Free PMC article.
-
Highway to hell-thy meiotic divisions: Chromosome passenger complex functions driven by microtubules: CPC interactions with both the chromosomes and microtubules are important for spindle assembly and function: CPC interactions with both the chromosomes and microtubules are important for spindle assembly and function.Bioessays. 2022 Jan;44(1):e2100202. doi: 10.1002/bies.202100202. Epub 2021 Nov 25. Bioessays. 2022. PMID: 34821405 Free PMC article.
References
-
- Adams RR, Wheatley SP, Gouldsworthy AM, Kandels-Lewis SE, Carmena M, Smythe C, Gerloff DL, Earnshaw WC. INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow. Curr Biol. 2000;10:1075–1078. - PubMed
-
- Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC. Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells. Chromosoma. 2001a;110:65–74. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases