Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation
- PMID: 16846377
- PMCID: PMC2777811
- DOI: 10.1089/scd.2006.15.407
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation
Abstract
Multipotent neural stem/progenitor cells (NSPCs) can be isolated from many regions of the adult central nervous system (CNS), yet neurogenesis is restricted to the hippocampus and subventricular zone in vivo. Identification of the molecular cues that modulate NSPC fate choice is a prerequisite for their therapeutic applications. Previously, we demonstrated that primary astrocytes isolated from regions with higher neuroplasticity, such as newborn and adult hippocampus and newborn spinal cord, promoted neuronal differentiation of adult NSPCs, whereas astrocytes isolated from the nonneurogenic region of the adult spinal cord inhibited neural differentiation. To identify the factors expressed by these astrocytes that could modulate NSPC differentiation, we performed gene expression profiling analysis using Affymetrix rat genome arrays. Our results demonstrated that these astrocytes had distinct gene expression profiles. We further tested the functional effects of candidate factors that were differentially expressed in neurogenesis-promoting and -inhibiting astrocytes using in vitro NSPC differentiation assays. Our results indicated that two interleukins, IL-1beta and IL-6, and a combination of factors that included these two interleukins could promote NSPC neuronal differentiation, whereas insulin-like growth factor binding protein 6 (IGFBP6) and decorin inhibited neuronal differentiation of adult NSPCs. Our results have provided further evidence to support the ongoing hypothesis that, in adult mammalian brains, astrocytes play critical roles in modulating NSPC differentiation. The finding that cytokines and chemokines expressed by astrocytes could promote NSPC neuronal differentiation may help us to understand how injuries induce neurogenesis in adult brains.
Figures
Similar articles
-
Gene expression profiling of human neural progenitor cells following the serum-induced astrocyte differentiation.Cell Mol Neurobiol. 2009 May;29(3):423-38. doi: 10.1007/s10571-008-9338-2. Epub 2009 Jan 7. Cell Mol Neurobiol. 2009. PMID: 19130216
-
The balance of Id3 and E47 determines neural stem/precursor cell differentiation into astrocytes.EMBO J. 2015 Nov 12;34(22):2804-19. doi: 10.15252/embj.201591118. Epub 2015 Oct 5. EMBO J. 2015. PMID: 26438726 Free PMC article.
-
Autocrine/paracrine platelet-derived growth factor regulates proliferation of neural progenitor cells.Cancer Res. 2006 Aug 15;66(16):8042-8. doi: 10.1158/0008-5472.CAN-06-0900. Cancer Res. 2006. PMID: 16912180
-
Role of IL-6 in the neural stem cell differentiation.Clin Rev Allergy Immunol. 2005 Jun;28(3):249-56. doi: 10.1385/CRIAI:28:3:249. Clin Rev Allergy Immunol. 2005. PMID: 16129909 Review.
-
Id proteins: emerging roles in CNS disease and targets for modifying neural stemcell behavior.Cell Tissue Res. 2022 Mar;387(3):433-449. doi: 10.1007/s00441-021-03490-z. Epub 2021 Jul 24. Cell Tissue Res. 2022. PMID: 34302526 Free PMC article. Review.
Cited by
-
Astrocyte-Neuron Interactions in Spinal Cord Injury.Adv Neurobiol. 2024;39:213-231. doi: 10.1007/978-3-031-64839-7_9. Adv Neurobiol. 2024. PMID: 39190077 Review.
-
Quiescent Adult Neural Stem Cells: Developmental Origin and Regulatory Mechanisms.Neurosci Bull. 2024 Apr 24. doi: 10.1007/s12264-024-01206-1. Online ahead of print. Neurosci Bull. 2024. PMID: 38656419 Review.
-
MLL1 inhibits the neurogenic potential of SCAPs by interacting with WDR5 and repressing HES1.Int J Oral Sci. 2023 Oct 18;15(1):48. doi: 10.1038/s41368-023-00253-0. Int J Oral Sci. 2023. PMID: 37852994 Free PMC article.
-
Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development.Neuron. 2023 Dec 20;111(24):3988-4005.e11. doi: 10.1016/j.neuron.2023.09.014. Epub 2023 Oct 10. Neuron. 2023. PMID: 37820724
-
Elevated levels of FMRP-target MAP1B impair human and mouse neuronal development and mouse social behaviors via autophagy pathway.Nat Commun. 2023 Jun 26;14(1):3801. doi: 10.1038/s41467-023-39337-0. Nat Commun. 2023. PMID: 37365192 Free PMC article.
References
-
- Bayer SA, Altman J, Russo RJ, Zhang X. Timetables of neurogenesis in the human brain based on experimentally determined patterns in the rat. Neurotoxicology. 1993;14:83–144. - PubMed
-
- Horner PJ, Palmer TD. New roles for astrocytes: the nightlife of an ‘astrocyte’. La vida loca! Trends Neurosci. 2003;26:597–603. - PubMed
-
- Zhao X, Schaffer D, Gage F. Stem cells in the Nervous System:Functional and Clinical Implications. Springer-Verlag; Berlin, Heidelberg: 2004.
-
- Ming GL, Song H. Adult neurogenesis in the mammalian central nervous system. Annu Rev Neurosci. 2005;28:223–50. - PubMed
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous