Toll-like receptors and their ligands control mesenchymal stem cell functions
- PMID: 17038530
- DOI: 10.1182/blood-2006-06-028704
Toll-like receptors and their ligands control mesenchymal stem cell functions
Abstract
Mesenchymal stem cells (MSCs) are widespread in adult organisms and may be involved in tissue maintenance and repair as well as in the regulation of hematopoiesis and immunologic responses. Thus, it is important to discover the factors controlling MSC renewal and differentiation. Here we report that adult MSCs express functional Toll-like receptors (TLRs), confirmed by the responses of MSCs to TLR ligands. Pam3Cys, a prototypic TLR-2 ligand, augmented interleukin-6 secretion by MSC, induced nuclear factor kappa B (NF-kappaB) translocation, reduced MSC basal motility, and increased MSC proliferation. The hallmark of MSC function is the capacity to differentiate into several mesodermal lineages. We show herein that Pam3Cys inhibited MSC differentiation into osteogenic, adipogenic, and chondrogenic cells while sparing their immunosuppressive effect. Our study therefore shows that a TLR ligand can antagonize MSC differentiation triggered by exogenous mediators and consequently maintains the cells in an undifferentiated and proliferating state in vitro. Moreover, MSCs derived from myeloid factor 88 (MyD88)-deficient mice lacked the capacity to differentiate effectively into osteogenic and chondrogenic cells. It appears that TLRs and their ligands can serve as regulators of MSC proliferation and differentiation and might affect the maintenance of MSC multipotency.
Similar articles
-
RelA promotes proliferation but inhibits osteogenic and chondrogenic differentiation of mesenchymal stem cells.FEBS Lett. 2020 May;594(9):1368-1378. doi: 10.1002/1873-3468.13739. Epub 2020 Feb 19. FEBS Lett. 2020. PMID: 31981416
-
Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation.Stem Cell Res Ther. 2018 Jul 4;9(1):178. doi: 10.1186/s13287-018-0933-y. Stem Cell Res Ther. 2018. PMID: 29973295 Free PMC article.
-
Toll-like receptors as a key regulator of mesenchymal stem cell function: An up-to-date review.Cell Immunol. 2017 May;315:1-10. doi: 10.1016/j.cellimm.2016.12.005. Epub 2016 Dec 26. Cell Immunol. 2017. PMID: 28284487 Review.
-
Mesenchymal stem cells: a perspective from in vitro cultures to in vivo migration and niches.Eur Cell Mater. 2010 Sep 1;20:121-33. doi: 10.22203/ecm.v020a11. Eur Cell Mater. 2010. PMID: 21249629 Review.
-
The role of BMP-7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro.J Cell Biochem. 2010 Feb 1;109(2):406-16. doi: 10.1002/jcb.22412. J Cell Biochem. 2010. PMID: 19950204
Cited by
-
Regulation of immune responses to infection through interaction between stem cell-derived exosomes and toll-like receptors mediated by microRNA cargoes.Front Cell Infect Microbiol. 2024 May 2;14:1384420. doi: 10.3389/fcimb.2024.1384420. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38756232 Free PMC article. Review.
-
Maintenance of homeostasis by TLR4 ligands.Front Immunol. 2024 Apr 23;15:1286270. doi: 10.3389/fimmu.2024.1286270. eCollection 2024. Front Immunol. 2024. PMID: 38715610 Free PMC article. Review.
-
Mesenchymal stem cells (MSCs) from the mouse bone marrow show differential expression of interferon regulatory factors IRF-1 and IRF-2.Mol Biol Rep. 2024 Jan 9;51(1):97. doi: 10.1007/s11033-023-09025-9. Mol Biol Rep. 2024. PMID: 38194130
-
Effects of histone acetyltransferase (HAT) and histone deacetylase (HDAC) inhibitors on proliferative, differentiative, and regenerative functions of Toll-like receptor 2 (TLR-2)-stimulated human dental pulp cells (hDPCs).Clin Oral Investig. 2023 Dec 29;28(1):53. doi: 10.1007/s00784-023-05466-5. Clin Oral Investig. 2023. PMID: 38157054
-
A new generation of mesenchymal stromal/stem cells differentially trained by immunoregulatory probiotics in a lupus microenvironment.Stem Cell Res Ther. 2023 Dec 10;14(1):358. doi: 10.1186/s13287-023-03578-z. Stem Cell Res Ther. 2023. PMID: 38072921 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases