Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast
- PMID: 1714605
- PMCID: PMC52310
- DOI: 10.1073/pnas.88.16.7434
Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast
Abstract
The ethylene-forming enzyme (EFE), which catalyzes the last step in the biosynthesis of the plant hormone ethylene, has never been purified and no molecular probes are available. Recently, a putative cDNA clone for tomato EFE (pTOM13) has been identified by inhibiting ethylene synthesis with an antisense gene expressed in transgenic plants. A direct test of its function has been made by expression of a pTOM13 gene in Saccharomyces cerevisiae. After cloning artefacts were discovered in the 5' region of the cDNA, a corrected cDNA (pRC13) was created by the fusion of the 5' end of a genomic clone to the 3' end of the cDNA and expressed in S. cerevisiae. Cultures of transformed yeast converted 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene, whereas control cells did not. This EFE activity displays similar characteristics to EFE found in plant tissue: it converts the trans isomer of the ACC analogue 1-amino-2-ethylcyclopropane-1-carboxylic acid to 1-butene in preference to the cis isomer, and it is strongly inhibited by cobaltous ions and 1,10-phenanthroline. Furthermore, information gained from the activity of effectors on yeast EFE activity supports the hypothesis that EFE is one of a group of hydroxylase enzymes.
Similar articles
-
Apple ripening-related cDNA clone pAP4 confers ethylene-forming ability in transformed Saccharomyces cerevisiae.Plant Physiol. 1993 Jul;102(3):783-8. doi: 10.1104/pp.102.3.783. Plant Physiol. 1993. PMID: 8278533 Free PMC article.
-
Analysis and cloning of the ethylene-forming enzyme from tomato by functional expression of its mRNA in Xenopus laevis oocytes.EMBO J. 1991 Aug;10(8):2007-13. doi: 10.1002/j.1460-2075.1991.tb07730.x. EMBO J. 1991. PMID: 2065651 Free PMC article.
-
Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants.Proc Natl Acad Sci U S A. 1989 Sep;86(17):6621-5. doi: 10.1073/pnas.86.17.6621. Proc Natl Acad Sci U S A. 1989. PMID: 2671999 Free PMC article.
-
Identification of genes for the ethylene-forming enzyme and inhibition of ethylene synthesis in transgenic plants using antisense genes.Biochem Soc Trans. 1992 Feb;20(1):76-9. doi: 10.1042/bst0200076. Biochem Soc Trans. 1992. PMID: 1633997 Review. No abstract available.
-
Biological formation of ethylene.RSC Chem Biol. 2023 Jul 10;4(9):635-646. doi: 10.1039/d3cb00066d. eCollection 2023 Aug 30. RSC Chem Biol. 2023. PMID: 37654506 Free PMC article. Review.
Cited by
-
Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants.Plants (Basel). 2024 Mar 11;13(6):791. doi: 10.3390/plants13060791. Plants (Basel). 2024. PMID: 38592775 Free PMC article. Review.
-
Diverse plant promoting bacterial species differentially improve tomato plant fitness under water stress.Front Plant Sci. 2023 Nov 24;14:1297090. doi: 10.3389/fpls.2023.1297090. eCollection 2023. Front Plant Sci. 2023. PMID: 38078116 Free PMC article.
-
Genome-Wide Identification and Expression Analysis of 1-Aminocyclopropane-1-Carboxylate Synthase (ACS) Gene Family in Chenopodium quinoa.Plants (Basel). 2023 Nov 29;12(23):4021. doi: 10.3390/plants12234021. Plants (Basel). 2023. PMID: 38068656 Free PMC article.
-
Activation of Gossypium hirsutum ACS6 Facilitates Fiber Development by Improving Sucrose Metabolism and Transport.Plants (Basel). 2023 Oct 11;12(20):3530. doi: 10.3390/plants12203530. Plants (Basel). 2023. PMID: 37895992 Free PMC article.
-
Elevated methylglyoxal levels inhibit tomato fruit ripening by preventing ethylene biosynthesis.Plant Physiol. 2023 Jul 3;192(3):2161-2184. doi: 10.1093/plphys/kiad142. Plant Physiol. 2023. PMID: 36879389 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources