Self-acylation properties of type II fatty acid biosynthesis acyl carrier protein
- PMID: 17656314
- DOI: 10.1016/j.chembiol.2007.05.013
Self-acylation properties of type II fatty acid biosynthesis acyl carrier protein
Abstract
Acyl carrier protein (ACP) plays a central role in many metabolic processes inside the cell, and almost 4% of the total enzymes inside the cell require it as a cofactor. Here, we report self-acylation properties in ACPs from Plasmodium falciparum and Brassica napus that are essential components of type II fatty acid biosynthesis (FAS II), disproving the existing notion that this phenomenon is restricted only to ACPs involved in polyketide biosynthesis. We also provide strong evidence to suggest that catalytic self-acylation is intrinsic to the individual ACP. Mutational analysis of these ACPs revealed the key residue(s) involved in this phenomenon. We also demonstrate that these FAS II ACPs exhibit a high degree of selectivity for self-acylation employing only dicarboxylic acids as substrates. A plausible mechanism for the self-acylation reaction is also proposed.
Similar articles
-
Catalysis and mechanism of malonyl transferase activity in type II fatty acid biosynthesis acyl carrier proteins.Mol Biosyst. 2009 Jun;5(6):651-9. doi: 10.1039/b820420a. Epub 2009 Apr 27. Mol Biosyst. 2009. PMID: 19462023
-
Evolutionary significance of self-acylation property in acyl carrier proteins.IUBMB Life. 2009 Aug;61(8):853-9. doi: 10.1002/iub.224. IUBMB Life. 2009. PMID: 19621347
-
Acyl carrier protein: structure-function relationships in a conserved multifunctional protein family.Biochem Cell Biol. 2007 Dec;85(6):649-62. doi: 10.1139/o07-109. Biochem Cell Biol. 2007. PMID: 18059524 Review.
-
Amino acid residues of Escherichia coli acyl carrier protein involved in heterologous protein interactions.Biochemistry. 2003 Jan 14;42(1):167-76. doi: 10.1021/bi0261950. Biochemistry. 2003. PMID: 12515551
-
Current understanding of fatty acid biosynthesis and the acyl carrier protein.Biochem J. 2010 Aug 15;430(1):1-19. doi: 10.1042/BJ20100462. Biochem J. 2010. PMID: 20662770 Review.
Cited by
-
Elucidation of unusual biosynthesis and DnaN-targeting mode of action of potent anti-tuberculosis antibiotics Mycoplanecins.Nat Commun. 2024 Jan 26;15(1):791. doi: 10.1038/s41467-024-44953-5. Nat Commun. 2024. PMID: 38278788 Free PMC article.
-
Genome-wide identification and analysis of ACP gene family in Sorghum bicolor (L.) Moench.BMC Genomics. 2022 Jul 25;23(1):538. doi: 10.1186/s12864-022-08776-2. BMC Genomics. 2022. PMID: 35879672 Free PMC article. Review.
-
Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways.J Biol Chem. 2021 Jan-Jun;296:100328. doi: 10.1016/j.jbc.2021.100328. Epub 2021 Jan 23. J Biol Chem. 2021. PMID: 33493513 Free PMC article. Review.
-
Acquisition of exogenous fatty acids renders apicoplast-based biosynthesis dispensable in tachyzoites of Toxoplasma.J Biol Chem. 2020 May 29;295(22):7743-7752. doi: 10.1074/jbc.RA120.013004. Epub 2020 Apr 27. J Biol Chem. 2020. PMID: 32341123 Free PMC article.
-
Biochemical characterization of the minimal domains of an iterative eukaryotic polyketide synthase.FEBS J. 2018 Dec;285(23):4494-4511. doi: 10.1111/febs.14675. Epub 2018 Oct 25. FEBS J. 2018. PMID: 30300504 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous