Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells
- PMID: 17686995
- PMCID: PMC1995710
- DOI: 10.1091/mbc.e07-02-0097
Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells
Abstract
The octameric exocyst complex is associated with the junctional complex and recycling endosomes and is proposed to selectively tether cargo vesicles directed toward the basolateral surface of polarized Madin-Darby canine kidney (MDCK) cells. We observed that the exocyst subunits Sec6, Sec8, and Exo70 were localized to early endosomes, transferrin-positive common recycling endosomes, and Rab11a-positive apical recycling endosomes of polarized MDCK cells. Consistent with its localization to multiple populations of endosomes, addition of function-blocking Sec8 antibodies to streptolysin-O-permeabilized cells revealed exocyst requirements for several endocytic pathways including basolateral recycling, apical recycling, and basolateral-to-apical transcytosis. The latter was selectively dependent on interactions between the small GTPase Rab11a and Sec15A and was inhibited by expression of the C-terminus of Sec15A or down-regulation of Sec15A expression using shRNA. These results indicate that the exocyst complex may be a multipurpose regulator of endocytic traffic directed toward both poles of polarized epithelial cells and that transcytotic traffic is likely to require Rab11a-dependent recruitment and modulation of exocyst function, likely through interactions with Sec15A.
Figures
![Figure 1.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520001.gif)
![Figure 2.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520002.gif)
![Figure 3.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520003.gif)
![Figure 4.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520004.gif)
![Figure 5.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520005.gif)
![Figure 6.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520006.gif)
![Figure 7.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520007.gif)
![Figure 8.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520008.gif)
![Figure 9.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520009.gif)
![Figure 10.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/1995710/bin/zmk0100782520010.gif)
Similar articles
-
Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells.Mol Biol Cell. 1999 Jan;10(1):47-61. doi: 10.1091/mbc.10.1.47. Mol Biol Cell. 1999. PMID: 9880326 Free PMC article.
-
Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25.J Biol Chem. 2000 Sep 15;275(37):29138-46. doi: 10.1074/jbc.M004410200. J Biol Chem. 2000. PMID: 10869360
-
Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells.J Cell Sci. 2001 Sep;114(Pt 18):3309-21. doi: 10.1242/jcs.114.18.3309. J Cell Sci. 2001. PMID: 11591819
-
TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells.Mol Biol Cell. 2014 Nov 15;25(23):3779-97. doi: 10.1091/mbc.E13-10-0604. Epub 2014 Sep 17. Mol Biol Cell. 2014. PMID: 25232007 Free PMC article.
-
New roles for endosomes: from vesicular carriers to multi-purpose platforms.Nat Rev Mol Cell Biol. 2009 Apr;10(4):287-92. doi: 10.1038/nrm2652. Epub 2009 Mar 11. Nat Rev Mol Cell Biol. 2009. PMID: 19277045 Free PMC article. Review.
Cited by
-
The exocyst complex is required for the trafficking and delivery of KCa3.1 to the basolateral membrane of polarized epithelia.Am J Physiol Cell Physiol. 2023 Jun 1;324(6):C1249-C1262. doi: 10.1152/ajpcell.00374.2022. Epub 2023 May 1. Am J Physiol Cell Physiol. 2023. PMID: 37125772 Free PMC article.
-
The exocyst complex is an essential component of the mammalian constitutive secretory pathway.J Cell Biol. 2023 May 1;222(5):e202205137. doi: 10.1083/jcb.202205137. Epub 2023 Mar 15. J Cell Biol. 2023. PMID: 36920342 Free PMC article.
-
Plasmolipin regulates basolateral-to-apical transcytosis of ICAM-1 and leukocyte adhesion in polarized hepatic epithelial cells.Cell Mol Life Sci. 2022 Jan 9;79(1):61. doi: 10.1007/s00018-021-04095-z. Cell Mol Life Sci. 2022. PMID: 34999972 Free PMC article.
-
Spatial bias in cAMP generation determines biological responses to PTH type 1 receptor activation.Sci Signal. 2021 Oct 5;14(703):eabc5944. doi: 10.1126/scisignal.abc5944. Epub 2021 Oct 5. Sci Signal. 2021. PMID: 34609896 Free PMC article.
-
RASSF8-mediated transport of Echinoid via the exocyst promotes Drosophila wing elongation and epithelial ordering.Development. 2021 Oct 15;148(20):dev199731. doi: 10.1242/dev.199731. Epub 2021 Oct 14. Development. 2021. PMID: 34532737 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
- R01 DK048370/DK/NIDDK NIH HHS/United States
- R01 DK054425/DK/NIDDK NIH HHS/United States
- DK-54425/DK/NIDDK NIH HHS/United States
- DK-54407/DK/NIDDK NIH HHS/United States
- DK-070856/DK/NIDDK NIH HHS/United States
- R01 DK043405/DK/NIDDK NIH HHS/United States
- NS0-38892/NS/NINDS NIH HHS/United States
- R37 DK054425/DK/NIDDK NIH HHS/United States
- R01 NS038892/NS/NINDS NIH HHS/United States
- Z01 DK043405/ImNIH/Intramural NIH HHS/United States
- R01 DK054407/DK/NIDDK NIH HHS/United States
- DK-48370/DK/NIDDK NIH HHS/United States
- R01 GM067002/GM/NIGMS NIH HHS/United States
- R01 DK051970/DK/NIDDK NIH HHS/United States
- GM-67002/GM/NIGMS NIH HHS/United States
- DK-43405/DK/NIDDK NIH HHS/United States
- R56 DK070856/DK/NIDDK NIH HHS/United States
- R01 DK070856/DK/NIDDK NIH HHS/United States
- DK-51970/DK/NIDDK NIH HHS/United States
LinkOut - more resources
Full Text Sources
Miscellaneous