Polarization-dependent selective transport to the apical membrane by KIF5B in MDCK cells
- PMID: 17925227
- PMCID: PMC3712496
- DOI: 10.1016/j.devcel.2007.08.001
Polarization-dependent selective transport to the apical membrane by KIF5B in MDCK cells
Abstract
Microtubule-based vesicular transport is well documented in epithelial cells, but the specific motors involved and their regulation during polarization are largely unknown. We demonstrate that KIF5B mediates post-Golgi transport of an apical protein in epithelial cells, but only after polarity has developed. Time-lapse imaging of EB1-GFP in polarized MDCK cells showed microtubule plus ends growing toward the apical membrane, implying that plus end-directed N-kinesins might be used to transport apical proteins. Indeed, time-lapse microscopy revealed that expression of a KIF5B dominant negative or microinjection of function-blocking KIF5 antibodies inhibited selectively post-Golgi transport of the apical marker, p75-GFP, after polarization of MDCK cells. Expression of other KIF dominant negatives did not alter p75-GFP trafficking. Immunoprecipitation experiments demonstrated an interaction between KIF5B and p75-GFP in polarized, but not in subconfluent, MDCK cells. Our results demonstrate that apical protein transport depends on selective microtubule motors and that epithelial cells switch kinesins for post-Golgi transport during acquisition of polarity.
Figures
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Comment in
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Catch the KIF5B train to the apical surface.Dev Cell. 2007 Oct;13(4):457-8. doi: 10.1016/j.devcel.2007.09.011. Dev Cell. 2007. PMID: 17925219
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References
-
- Bonifacino JS, Traub LM. Signals for sorting of trans-membrane proteins to endosomes and lysosomes. Annu Rev Biochem. 2003;72:395–447. - PubMed
-
- Brady ST, Lasek RJ, Allen RD. Fast axonal transport in extruded axoplasm from squid giant axon. Science. 1982;218:1129–1131. - PubMed
-
- Bu W, Su LK. Regulation of microtubule assembly by human EB1 family proteins. Oncogene. 2001;20:3185–3192. - PubMed
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