A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes
- PMID: 18723671
- PMCID: PMC2519042
- DOI: 10.1073/pnas.0805118105
A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes
Abstract
Although much has been learned about short noncoding RNAs, long noncoding transcripts are largely uncharacterized. Here, we describe Caenorhabditis elegans rncs-1, a highly base-paired, 800-nucleotide noncoding RNA expressed in hypodermis and intestine. Transcription of rncs-1 is modulated in response to food supply. Although highly double-stranded, we show that rncs-1 RNA is not a substrate for Dicer because of branched structures at its termini. However, rncs-1 RNA inhibits Dicer cleavage of a second dsRNA in vitro, presumably by competition. We validate this observation in vivo by demonstrating that mRNA levels of several Dicer-regulated genes vary with changes in rncs-1 expression. Certain viruses express dsRNA to compete with cellular dsRNA-mediated pathways, and our data suggest that rncs-1 provides a cellular correlate of this phenomenon.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
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References
-
- Frith MC, Pheasant M, Mattick JS. The amazing complexity of the human transcriptome. Eur J Hum Genet. 2005;13:894–897. - PubMed
-
- Prasanth KV, Spector DL. Eukaryotic regulatory RNAs: An answer to the “genome complexity” conundrum. Genes Dev. 2007;21:11–42. - PubMed
-
- Mattick JS, Makunin IV. Noncoding RNA. Hum Mol Genet. 2006;15:R17–R29. - PubMed
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