Chromatin-bound mitogen-activated protein kinases transmit dynamic signals in transcription complexes in beta-cells
- PMID: 18755896
- PMCID: PMC2533187
- DOI: 10.1073/pnas.0806465105
Chromatin-bound mitogen-activated protein kinases transmit dynamic signals in transcription complexes in beta-cells
Abstract
MAPK pathways regulate transcription through phosphorylation of transcription factors and other DNA-binding proteins. In pancreatic beta-cells, ERK1/2 are required for transcription of the insulin gene and several other genes in response to glucose. We show that binding of glucose-sensitive transcription activators and repressors to the insulin gene promoter depends on ERK1/2 activity. We also find that glucose and NGF stimulate the binding of ERK1/2 to the insulin gene and other promoters. An ERK1/2 cascade module, including MEK1/2 and Rsk, are found in complexes bound to these promoters. These findings imply that MAPK-containing signaling complexes are positioned on sensitive promoters with their protein substrates to modulate transcription in situ in response to incoming signals.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
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![Fig. 4.](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2533187/bin/zpq9990845680004.gif)
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