Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles
- PMID: 1887589
- PMCID: PMC7131244
- DOI: 10.1016/0042-6822(91)90437-g
Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles
Abstract
The major subgenomic RNA of the calicivirus rabbit hemorrhagic disease virus which codes for the viral capsid protein has been cloned as cDNA. The nucleotide sequence of this mRNA was shown to be identical to the 3' terminal region of the genomic RNA. The 5' end of the mRNA corresponds to position 5296 of the genomic sequence; except for two differences the first 16 nucleotides of genomic and subgenomic RNAs are identical. After isolation from liver tissue viral genomic and subgenomic RNAs were found to be resistant to RNase degradation. This protection was due to RNA packaging into particles. Sucrose density gradient centrifugation of liver homogenates allowed separation of such particles containing either genomic RNA or subgenomic RNA. Genomic and subgenomic RNAs are protein-linked and for the genomic molecule this interaction is localized within the first 179 nucleotides. After radioactive labeling of purified RNA and subsequent RNase treatment a protein of 15 kDa was identified.
Similar articles
-
Synthesis in vitro of rabbit hemorrhagic disease virus subgenomic RNA by internal initiation on (-)sense genomic RNA: mapping of a subgenomic promoter.J Biol Chem. 2004 Apr 23;279(17):17013-8. doi: 10.1074/jbc.M313674200. Epub 2004 Jan 26. J Biol Chem. 2004. PMID: 14744857
-
Detection of the ORF3 polypeptide of feline calicivirus in infected cells and evidence for its expression from a single, functionally bicistronic, subgenomic mRNA.J Gen Virol. 1996 Jan;77 ( Pt 1):123-7. doi: 10.1099/0022-1317-77-1-123. J Gen Virol. 1996. PMID: 8558120
-
Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome.Virology. 1991 Oct;184(2):664-76. doi: 10.1016/0042-6822(91)90436-f. Virology. 1991. PMID: 1840711
-
Identification of the viral haemorrhagic disease virus of rabbits as a calicivirus.Rev Sci Tech. 1991 Jun;10(2):311-23. doi: 10.20506/rst.10.2.548. Rev Sci Tech. 1991. PMID: 1662097 Review.
-
Haemorrhagic disease of lagomorphs: evidence for a calicivirus.Vet Microbiol. 1992 Nov;33(1-4):375-81. doi: 10.1016/0378-1135(92)90065-2. Vet Microbiol. 1992. PMID: 1282757 Review.
Cited by
-
First Detection and Circulation of RHDV2 in New Zealand.Viruses. 2024 Mar 28;16(4):519. doi: 10.3390/v16040519. Viruses. 2024. PMID: 38675862 Free PMC article.
-
First Detection of Benign Rabbit Caliciviruses in Chile.Viruses. 2024 Mar 12;16(3):439. doi: 10.3390/v16030439. Viruses. 2024. PMID: 38543804 Free PMC article.
-
Recombination between non-structural and structural genes as a mechanism of selection in lagoviruses: The evolutionary dead-end of an RHDV2 isolated from European hare.Virus Res. 2024 Jan 2;339:199257. doi: 10.1016/j.virusres.2023.199257. Epub 2023 Nov 3. Virus Res. 2024. PMID: 38347757 Free PMC article.
-
Utilizing Molecular Epidemiology and Citizen Science for the Surveillance of Lagoviruses in Australia.Viruses. 2023 Nov 29;15(12):2348. doi: 10.3390/v15122348. Viruses. 2023. PMID: 38140589 Free PMC article.
-
Lagovirus Non-structural Protein p23: A Putative Viroporin That Interacts With Heat Shock Proteins and Uses a Disulfide Bond for Dimerization.Front Microbiol. 2022 Jul 7;13:923256. doi: 10.3389/fmicb.2022.923256. eCollection 2022. Front Microbiol. 2022. PMID: 35923397 Free PMC article.
References
-
- Beaucage S.L., Caruthers M.H. Deoxynucleoside phosphoramidites: Anew class of key intermediates for deoxynucleotide synthesis. Tetrahedron Lett. 1981;22:1859–1862.
-
- Black D.N., Burroughs J.N., Harris T.J.R., Brown F. The structure and replication of calicivirus RNA. Nature. 1978;274:614–616. - PubMed
-
- Burroughs J.N., Brown F. Presence of a covalently linked protein on caliciviral RNA. J. Gen. Virol. 1978;41:443–446. - PubMed
-
- Chirgwin J.M., Przybyla A.E., MacDonald R.J., Rutter W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979;18:5294–5299. - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources