Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts
- PMID: 19041755
- DOI: 10.1016/j.cell.2008.09.040
Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts
Abstract
Epithelial cells contain noncentrosomal microtubules (MTs), whose minus ends are oriented apically. In contrast with the well-known interactions of the minus ends with the centrosome, little is known about the termination site of the noncentrosomal minus ends. Here we show that a population of MT minus ends is anchored at the zonula adherens (ZA), the apical-most part of the cadherin-based adherens junction, via a protein that we have termed Nezha. We initially identified PLEKHA7 as a ZA component and subsequently detected Nezha as a partner for PLEKHA7. Nezha bound MTs at their minus ends and tethered them to the ZA. Furthermore, we found that a minus end-directed motor, KIFC3, was concentrated at the ZA in a PLEKHA7/Nezha/MT-dependent manner; and depletion of any of these proteins resulted in disorganization of the ZA. We propose that the PLEKHA7/Nezha/MT complex regulates the ZA integrity by recruiting KIFC3 to this junctional site.
Comment in
-
Organizing junctions at the cell-cell interface.Cell. 2008 Nov 28;135(5):791-3. doi: 10.1016/j.cell.2008.11.002. Cell. 2008. PMID: 19041742
Similar articles
-
A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells.J Biol Chem. 2011 May 13;286(19):16743-50. doi: 10.1074/jbc.M111.230862. Epub 2011 Mar 21. J Biol Chem. 2011. PMID: 21454477 Free PMC article.
-
Binding between the junctional proteins afadin and PLEKHA7 and implication in the formation of adherens junction in epithelial cells.J Biol Chem. 2013 Oct 11;288(41):29356-68. doi: 10.1074/jbc.M113.453464. Epub 2013 Aug 29. J Biol Chem. 2013. PMID: 23990464 Free PMC article.
-
PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.Cell Cycle. 2016;15(4):498-505. doi: 10.1080/15384101.2016.1141840. Cell Cycle. 2016. PMID: 26822694 Free PMC article.
-
PLEKHA7: Cytoskeletal adaptor protein at center stage in junctional organization and signaling.Int J Biochem Cell Biol. 2016 Jun;75:112-6. doi: 10.1016/j.biocel.2016.04.001. Epub 2016 Apr 9. Int J Biochem Cell Biol. 2016. PMID: 27072621 Review.
-
A central role for cadherin signaling in cancer.Exp Cell Res. 2017 Sep 1;358(1):78-85. doi: 10.1016/j.yexcr.2017.04.006. Epub 2017 Apr 12. Exp Cell Res. 2017. PMID: 28412244 Free PMC article. Review.
Cited by
-
Deficiency of CAMSAP2 impairs olfaction and the morphogenesis of mitral cells.EMBO Rep. 2024 Jul;25(7):2861-2877. doi: 10.1038/s44319-024-00166-x. Epub 2024 Jun 5. EMBO Rep. 2024. PMID: 38839944 Free PMC article.
-
CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC.Nat Cell Biol. 2024 Mar;26(3):404-420. doi: 10.1038/s41556-024-01366-2. Epub 2024 Feb 29. Nat Cell Biol. 2024. PMID: 38424271 Free PMC article.
-
Patronin regulates presynaptic microtubule organization and neuromuscular junction development in Drosophila.iScience. 2024 Jan 18;27(2):108944. doi: 10.1016/j.isci.2024.108944. eCollection 2024 Feb 16. iScience. 2024. PMID: 38318379 Free PMC article.
-
Crystal structure of the Arabidopsis SPIRAL2 C-terminal domain reveals a p80-Katanin-like domain.PLoS One. 2023 Dec 29;18(12):e0290024. doi: 10.1371/journal.pone.0290024. eCollection 2023. PLoS One. 2023. PMID: 38157339 Free PMC article.
-
CAMSAP1 role in orchestrating structure and dynamics of manchette microtubule minus-ends impacts male fertility during spermiogenesis.Proc Natl Acad Sci U S A. 2023 Nov 7;120(45):e2313787120. doi: 10.1073/pnas.2313787120. Epub 2023 Oct 30. Proc Natl Acad Sci U S A. 2023. PMID: 37903275 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases