Membrane binding by tBid initiates an ordered series of events culminating in membrane permeabilization by Bax
- PMID: 19062087
- DOI: 10.1016/j.cell.2008.11.010
Membrane binding by tBid initiates an ordered series of events culminating in membrane permeabilization by Bax
Abstract
In normal circumstances, the Bcl-2 family dutifully governs when cells die. However, the rules of engagement between the pro- and antiapoptotic family members are still contested, and how Bax is transformed from a cytosolic monomer to an outer mitochondrial membrane-permeabilizing oligomer is unclear. With fluorescence techniques and an in vitro system, the combination of tBid and Bax produced dramatic membrane permeabilization. The membrane is not a passive partner in this process beause membranes are required for the protein-protein interactions to occur. Simultaneous measurements of these interactions revealed an ordered series of steps required for outer membrane permeabilization: (1) tBid rapidly binds to membranes, where (2) tBid interacts with Bax, causing (3) Bax insertion into membranes and (4) oligomerization, culminating in (5) membrane permeabilization. Bcl-XL prevents membrane-bound tBid from binding Bax. Bad releases tBid from Bcl-XL, restoring both tBid binding to Bax and membrane permeabilization.
Comment in
-
Bcl-2 proteins and apoptosis: choose your partner.Cell. 2008 Dec 12;135(6):1004-6. doi: 10.1016/j.cell.2008.11.029. Cell. 2008. PMID: 19070569
Similar articles
-
Bax oligomerization in mitochondrial membranes requires tBid (caspase-8-cleaved Bid) and a mitochondrial protein.Biochem J. 2002 Dec 15;368(Pt 3):915-21. doi: 10.1042/BJ20020972. Biochem J. 2002. PMID: 12193163 Free PMC article.
-
Mitochondrial targeting of tBid/Bax: a role for the TOM complex?Cell Death Differ. 2009 Aug;16(8):1075-82. doi: 10.1038/cdd.2009.61. Epub 2009 Jun 12. Cell Death Differ. 2009. PMID: 19521421 Review.
-
Distinct lipid effects on tBid and Bim activation of membrane permeabilization by pro-apoptotic Bax.Biochem J. 2015 May 1;467(3):495-505. doi: 10.1042/BJ20141291. Biochem J. 2015. PMID: 25714678
-
Contributions to Bax insertion and oligomerization of lipids of the mitochondrial outer membrane.Cell Death Differ. 2008 May;15(5):929-37. doi: 10.1038/cdd.2008.9. Cell Death Differ. 2008. PMID: 18259190
-
Life, death, BH3 profiles, and the salmon mousse.Cancer Cell. 2007 Aug;12(2):97-9. doi: 10.1016/j.ccr.2007.07.011. Cancer Cell. 2007. PMID: 17692799 Review.
Cited by
-
The C-terminal sequences of Bcl-2 family proteins mediate interactions that regulate cell death.Biochem J. 2024 Jul 17;481(14):903-922. doi: 10.1042/BCJ20210352. Biochem J. 2024. PMID: 38985308 Free PMC article. Review.
-
Lipid unsaturation promotes BAX and BAK pore activity during apoptosis.Nat Commun. 2024 Jun 3;15(1):4700. doi: 10.1038/s41467-024-49067-6. Nat Commun. 2024. PMID: 38830851 Free PMC article.
-
Directly targeting BAX for drug discovery: Therapeutic opportunities and challenges.Acta Pharm Sin B. 2024 Jun;14(6):2378-2401. doi: 10.1016/j.apsb.2024.02.010. Epub 2024 Feb 10. Acta Pharm Sin B. 2024. PMID: 38828138 Free PMC article. Review.
-
PAQR4 oncogene: a novel target for cancer therapy.Med Oncol. 2024 May 20;41(6):161. doi: 10.1007/s12032-024-02382-w. Med Oncol. 2024. PMID: 38767705 Review.
-
N6-Methyladenosine Methylation of mRNA in Cell Apoptosis.Mol Neurobiol. 2024 Jul;61(7):3934-3948. doi: 10.1007/s12035-023-03813-x. Epub 2023 Dec 2. Mol Neurobiol. 2024. PMID: 38040996 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
