Absorption measurements of cell monolayers relevant to mechanisms of laser phototherapy: reduction or oxidation of cytochrome c oxidase under laser radiation at 632.8 nm
- PMID: 19099388
- DOI: 10.1089/pho.2008.2246
Absorption measurements of cell monolayers relevant to mechanisms of laser phototherapy: reduction or oxidation of cytochrome c oxidase under laser radiation at 632.8 nm
Abstract
Objective: The objective of this work was a further investigation of redox mechanisms of laser phototherapy on the cellular level.
Background data: Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, is believed to work as the photoacceptor to modulate cellular metabolism in laser phototherapy.
Materials and methods: The changes in the absorption spectra of HeLa-cell monolayers before and after irradiation at 632.8 nm using fast multi-channel recording were evaluated by the intensity ratio between the peaks at 770 and 670 nm (intensity ratio criterion).
Results: By the intensity ratio criterion, the irradiation effects (reduction or oxidation of the photoacceptor) depended on the initial redox status of cytochrome c oxidase. The irradiation (three times at 632.8 nm, dose = 6.3 x 103 J/m(2), tau(irrad.) = 10 sec, tau(record.) = 600 msec) of cells initially characterized by relatively oxidized cytochrome c oxidase caused first a reduction of the photoacceptor, and then its oxidation (a bell-shaped curve). The irradiation by the same scheme of the cells with initially relatively reduced cytochrome c oxidase caused first oxidation and then a slight reduction of the enzyme (a curve opposite to the bell-shaped curve).
Conclusion: The experimental results of our work demonstrate that irradiation at 632.8 nm causes either a (transient) relative reduction of the photoacceptor, putatively cytochrome c oxidase, or its (transient) relative oxidation, depending on the initial redox status of the photoacceptor. The maximum in the bell-shaped dose-dependence curve or the minimum of the reverse curve is the turning point between the prevailing of oxidation or reduction processes. Our results are evidence that the bell-shaped dose dependences recorded for various cellular responses are characteristic also for redox changes in the photoacceptor, cytochrome c oxidase.
Similar articles
-
Mechanisms and Mitochondrial Redox Signaling in Photobiomodulation.Photochem Photobiol. 2018 Mar;94(2):199-212. doi: 10.1111/php.12864. Epub 2018 Jan 19. Photochem Photobiol. 2018. PMID: 29164625 Free PMC article. Review.
-
Mitochondrial signaling in mammalian cells activated by red and near-IR radiation.Photochem Photobiol. 2008 Sep-Oct;84(5):1091-9. doi: 10.1111/j.1751-1097.2008.00394.x. Epub 2008 Jul 18. Photochem Photobiol. 2008. PMID: 18651871 Review.
-
Photoreactions of cytochrome C oxidase.Photochem Photobiol. 2006 May-Jun;82(3):711-9. doi: 10.1562/2005-09-14-RA-684. Photochem Photobiol. 2006. PMID: 16789843
-
Absorption measurements of a cell monolayer relevant to phototherapy: reduction of cytochrome c oxidase under near IR radiation.J Photochem Photobiol B. 2005 Nov 1;81(2):98-106. doi: 10.1016/j.jphotobiol.2005.07.002. Epub 2005 Aug 26. J Photochem Photobiol B. 2005. PMID: 16125966
-
[Cytochrome c oxidase as the primary photoacceptor upon laser exposure of cultured cells to visible and near IR-range light].Dokl Akad Nauk. 1995 Jun;342(5):693-5. Dokl Akad Nauk. 1995. PMID: 7670387 Russian. No abstract available.
Cited by
-
Outcomes of whole-body photobiomodulation on pain, quality of life, leisure physical activity, pain catastrophizing, kinesiophobia, and self-efficacy: a prospective randomized triple-blinded clinical trial with 6 months of follow-up.Front Neurosci. 2024 Jan 31;18:1264821. doi: 10.3389/fnins.2024.1264821. eCollection 2024. Front Neurosci. 2024. PMID: 38356644 Free PMC article.
-
Repeated Low-Level Red-Light Therapy for Controlling Onset and Progression of Myopia-a Review.Int J Med Sci. 2023 Sep 4;20(10):1363-1376. doi: 10.7150/ijms.85746. eCollection 2023. Int J Med Sci. 2023. PMID: 37786442 Free PMC article. Review.
-
Effects of Violet and IR LED Light on mast cell degranulation: in vivo study in a murine model.Lasers Med Sci. 2023 Jul 13;38(1):159. doi: 10.1007/s10103-023-03825-y. Lasers Med Sci. 2023. PMID: 37442837
-
Photobiomodulation for Alzheimer's disease: photoelectric coupling effect on attenuating Aβ neurotoxicity.Lasers Med Sci. 2023 Jan 12;38(1):39. doi: 10.1007/s10103-022-03692-z. Lasers Med Sci. 2023. PMID: 36633696 Free PMC article. Review.
-
Changes in Circadian Variations in Blood Pressure, Pain Pressure Threshold and the Elasticity of Tissue after a Whole-Body Photobiomodulation Treatment in Patients with Fibromyalgia: A Tripled-Blinded Randomized Clinical Trial.Biomedicines. 2022 Oct 23;10(11):2678. doi: 10.3390/biomedicines10112678. Biomedicines. 2022. PMID: 36359198 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources