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. 2009 May;106(5):1705-14.
doi: 10.1111/j.1365-2672.2008.04140.x. Epub 2009 Feb 16.

Screening and characterization of monoclonal antibodies to the surface antigens of Listeria monocytogenes serotype 4b

M Lin  1 , S Armstrong, J Ronholm, H Dan, M-E Auclair, Z Zhang, X Cao
Affiliations
Free article

Screening and characterization of monoclonal antibodies to the surface antigens of Listeria monocytogenes serotype 4b

M Lin et al. J Appl Microbiol. 2009 May.
Free article

Abstract

Aims: This study aims to develop and characterize monoclonal antibodies (Mabs) with high specificity and affinity for surface antigens of an epidemiologically important serotype 4b of Listeria monocytogenes.

Methods and results: Hybridoma clones were derived from B lymphocytes of mice immunized with L. monocytogenes serotype 4b and screened against this strain by an enzyme-linked immunosorbent assay. Twenty-nine clones secreting Mabs reactive with formalin-killed bacteria were obtained; 15, 8, 5 and 1 Mabs were immunoglobulin subclasses IgG2a, IgG2b, IgM and IgG1, respectively. Immunofluorescence or immunogold labelling demonstrated all except five IgM and one IgG2a Mabs bound to the surface of a live L. monocytogenes serotype 4b. The majority of the 23 surface-binding Mabs recognized linear epitopes on a 77-kDa protein. These surface-binding Mabs exhibited little or no cross-reactivity with non-4b serotypes (1/2a, 1/2b, 3a, etc.) of L. monocytogenes, five other Listeria species, Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium.

Conclusions: The Mabs recognizing a 77-kDa surface protein are novel antibodies with specificity and affinity for L. monocytogenes serotype 4b.

Significance and impact of the study: These anti-77 kDa surface protein Mabs may be explored as reagents for the development of Mabs-based diagnostic immunoassays for L. monocytogenes serotype 4b strains.

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