System-wide changes to SUMO modifications in response to heat shock
- PMID: 19471022
- DOI: 10.1126/scisignal.2000282
System-wide changes to SUMO modifications in response to heat shock
Abstract
Covalent conjugation of the small ubiquitin-like modifier (SUMO) proteins to target proteins regulates many important eukaryotic cellular mechanisms. Although the molecular consequences of the conjugation of SUMO proteins are relatively well understood, little is known about the cellular signals that regulate the modification of their substrates. Here, we show that SUMO-2 and SUMO-3 are required for cells to survive heat shock. Through quantitative labeling techniques, stringent purification of SUMOylated proteins, advanced mass spectrometric technology, and novel techniques of data analysis, we quantified heat shock-induced changes in the SUMOylation state of 766 putative substrates. In response to heat shock, SUMO was polymerized into polySUMO chains and redistributed among a wide range of proteins involved in cell cycle regulation; apoptosis; the trafficking, folding, and degradation of proteins; transcription; translation; and DNA replication, recombination, and repair. This comprehensive proteomic analysis of the substrates of a ubiquitin-like modifier (Ubl) identifies a pervasive role for SUMO proteins in the biologic response to hyperthermic stress.
Similar articles
-
Heat shock transcription factor 1 is SUMOylated in the activated trimeric state.J Biol Chem. 2021 Jan-Jun;296:100324. doi: 10.1016/j.jbc.2021.100324. Epub 2021 Jan 23. J Biol Chem. 2021. PMID: 33493517 Free PMC article.
-
Small ubiquitin-like modifier 3-modified proteome regulated by brain ischemia in novel small ubiquitin-like modifier transgenic mice: putative protective proteins/pathways.Stroke. 2014 Apr;45(4):1115-22. doi: 10.1161/STROKEAHA.113.004315. Epub 2014 Feb 25. Stroke. 2014. PMID: 24569813 Free PMC article.
-
Cystic fibrosis transmembrane conductance regulator degradation: cross-talk between the ubiquitylation and SUMOylation pathways.FEBS J. 2013 Sep;280(18):4430-8. doi: 10.1111/febs.12415. Epub 2013 Jul 22. FEBS J. 2013. PMID: 23809253 Free PMC article. Review.
-
Mass spectrometric identification of SUMO substrates provides insights into heat stress-induced SUMOylation in plants.Plant Signal Behav. 2011 Jan;6(1):130-3. doi: 10.4161/psb.6.1.14256. Epub 2011 Jan 1. Plant Signal Behav. 2011. PMID: 21270536 Free PMC article. Review.
-
Proteomic revelation: SUMO changes partners when the heat is on.Sci Signal. 2009 Jul 28;2(81):pe45. doi: 10.1126/scisignal.281pe45. Sci Signal. 2009. PMID: 19638612 Free PMC article.
Cited by
-
Cold-induced FOXO1 nuclear transport aids cold survival and tissue storage.Nat Commun. 2024 Apr 3;15(1):2859. doi: 10.1038/s41467-024-47095-w. Nat Commun. 2024. PMID: 38570500 Free PMC article.
-
Unwinding Helicase MCM Functionality for Diagnosis and Therapeutics of Replication Abnormalities Associated with Cancer: A Review.Mol Diagn Ther. 2024 May;28(3):249-264. doi: 10.1007/s40291-024-00701-5. Epub 2024 Mar 26. Mol Diagn Ther. 2024. PMID: 38530633 Review.
-
Hypoxia-driven deSUMOylation of EXOSC10 promotes adaptive changes in the transcriptome profile.Cell Mol Life Sci. 2024 Jan 27;81(1):58. doi: 10.1007/s00018-023-05035-9. Cell Mol Life Sci. 2024. PMID: 38279024 Free PMC article.
-
SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cells.Cell Mol Biol Lett. 2024 Jan 16;29(1):15. doi: 10.1186/s11658-024-00533-5. Cell Mol Biol Lett. 2024. PMID: 38229033 Free PMC article.
-
Biological Parts for Engineering Abiotic Stress Tolerance in Plants.Biodes Res. 2022 Jan 21;2022:9819314. doi: 10.34133/2022/9819314. eCollection 2022. Biodes Res. 2022. PMID: 37850130 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources