Biochemical evidence of atherosclerosis progression mediated by increased oxidative stress in apolipoprotein E-deficient spontaneously hyperlipidemic mice exposed to chronic cigarette smoke
- PMID: 19571461
- DOI: 10.1254/jphs.09100fp
Biochemical evidence of atherosclerosis progression mediated by increased oxidative stress in apolipoprotein E-deficient spontaneously hyperlipidemic mice exposed to chronic cigarette smoke
Abstract
Cigarette smoking is a major risk factor for cardiovascular disease. The induction of oxidative stress by smoking plays a key role in the progression of atherosclerosis. However, the underlying mechanisms are not fully understood. In the present study, we investigated whether long-term smoking can accelerate the progression of atherosclerosis and whether oxidative stress is implicated in its pathogenesis. Apolipoprotein E-deficient spontaneously hyperlipidemic mice, a model of atherosclerosis, were exposed to the gas-phase of smoke, from which tar and nicotine had been removed, for 15 min a day, 6 days a week, for 16 weeks. Exposure to cigarette smoke significantly increased the serum levels of oxidative stress markers such as thiobarbituric acid-reactive substances, oxidatively modified low-density lipoproteins, and 3-nitrotyrosine, but it did not affect serum cholesterol and triglyceride levels. Exposure to smoke also accelerated the accumulation of total cholesterol levels in the aorta that was accompanied by an increase in 3-nitrotyrosine levels of the atherosclerotic mice. These changes in the serum and aorta that progressed with exposure to smoke were prevented by vitamin E administration. Our data suggest that chronic cigarette smoking promotes and aggravates atherosclerosis and that the antioxidant vitamin E exerts an anti-atherogenic effect via reduction of oxidative stress.
Similar articles
-
A 6-month systems toxicology inhalation study in ApoE-/- mice demonstrates reduced cardiovascular effects of E-vapor aerosols compared with cigarette smoke.Am J Physiol Heart Circ Physiol. 2020 Mar 1;318(3):H604-H631. doi: 10.1152/ajpheart.00613.2019. Epub 2020 Jan 24. Am J Physiol Heart Circ Physiol. 2020. PMID: 31975625
-
The Apoe(-/-) mouse model: a suitable model to study cardiovascular and respiratory diseases in the context of cigarette smoke exposure and harm reduction.J Transl Med. 2016 May 20;14(1):146. doi: 10.1186/s12967-016-0901-1. J Transl Med. 2016. PMID: 27207171 Free PMC article. Review.
-
[Oxidative stress and atherosclerosis].Yakugaku Zasshi. 2007 Dec;127(12):1997-2014. doi: 10.1248/yakushi.127.1997. Yakugaku Zasshi. 2007. PMID: 18057788 Review. Japanese.
-
Potentially reduced exposure cigarettes accelerate atherosclerosis: evidence for the role of nicotine.Cardiovasc Toxicol. 2007;7(3):192-201. doi: 10.1007/s12012-007-0027-z. Cardiovasc Toxicol. 2007. PMID: 17901562
-
Oxidants in cigarette smoke extract modify low-density lipoprotein in the plasma and facilitate atherogenesis in the aorta of Watanabe heritable hyperlipidemic rabbits.Atherosclerosis. 2001 May;156(1):109-17. doi: 10.1016/s0021-9150(00)00637-7. Atherosclerosis. 2001. PMID: 11369003
Cited by
-
Melatonin alleviates pyroptosis by regulating the SIRT3/FOXO3α/ROS axis and interacting with apoptosis in Atherosclerosis progression.Biol Res. 2023 Dec 2;56(1):62. doi: 10.1186/s40659-023-00479-6. Biol Res. 2023. PMID: 38041171 Free PMC article.
-
Smoking and the Pathophysiology of Peripheral Artery Disease.Front Cardiovasc Med. 2021 Aug 27;8:704106. doi: 10.3389/fcvm.2021.704106. eCollection 2021. Front Cardiovasc Med. 2021. PMID: 34513948 Free PMC article. Review.
-
Impact of whole-body versus nose-only inhalation exposure systems on systemic, respiratory, and cardiovascular endpoints in a 2-month cigarette smoke exposure study in the ApoE-/- mouse model.J Appl Toxicol. 2021 Oct;41(10):1598-1619. doi: 10.1002/jat.4149. Epub 2021 Apr 6. J Appl Toxicol. 2021. PMID: 33825214 Free PMC article.
-
Nicotine in Senescence and Atherosclerosis.Cells. 2020 Apr 22;9(4):1035. doi: 10.3390/cells9041035. Cells. 2020. PMID: 32331221 Free PMC article. Review.
-
Post-diagnosis smoking and risk of cardiovascular, cancer, and all-cause mortality in survivors of 10 adult cancers: a prospective cohort study.Am J Cancer Res. 2019 Nov 1;9(11):2493-2514. eCollection 2019. Am J Cancer Res. 2019. PMID: 31815049 Free PMC article.
