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. 2008 Sep;3(9):733-6.
doi: 10.4161/psb.3.9.6489.

Cell wall remodeling in Arabidopsis stamen abscission zones: Temporal aspects of control inferred from transcriptional profiling

Coralie C Lashbrook  1 , Suqin Cai
Affiliations

Cell wall remodeling in Arabidopsis stamen abscission zones: Temporal aspects of control inferred from transcriptional profiling

Coralie C Lashbrook et al. Plant Signal Behav. 2008 Sep.

Abstract

Organ shedding requires cell separation within abscission zones (AZs). Functional genomic AZ studies have been limited by their small size and low incidence. Optimization of laser capture microdissection (LCM) for AZs and other specialized cell types in Arabidopsis1 allowed recent characterization of the floral stamen AZ transcriptome responding to a developmental shedding cue.2 Analyses focused on 551 AZ transcripts (AZ(551)) that were regulated at the highest statistical significance (p </= 0.0001) over five stages of stamen development spanning pre-pollination to organ shed.2 Here, we seek a fuller understanding of AZ integrity control by relaxing P value restrictions on statistical significance ten-fold to generate an expanded population of 1461 stamen transcripts (AZ(1461)). Cell wall remodeling functions in AZ(1461) are significantly over-represented relative to all transcripts represented on the whole genome GeneChip. Hierarchical clustering of gene expression data corresponding to cell wall-related transcripts suggests a temporal model for AZ remodeling in Arabidopsis stamens destined to abscise.

Keywords: 4-glucanase; abscission zone; cell wall; endo-β-1; expansin; laser capture microdissection; pectate lyase; pectin methylesterase; polygalacturonase; stamen; xyloglucan endotransglucosylase/hydrolase.

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Figures

Figure 1
Figure 1
Developmental expression profiles of cell wall related genes within AZ1461. Transcripts were subjected to divisive hierarchical clustering. Enzyme families are further described on the Carbohydrate-Active Enzymes website (www.cazy.org) described by Coutinho et al. Floral stage numbers are defined elsewhere and in Figure 2. EXP expansin, EXPL expansin-like, GH9 glycosyl hydrolase family 9, GH17 glycosyl hydrolase family 17, PAE pectin acetylesterase, PG polygalacturonase, PL pectate lyase, PME pectin methylesterase, PMEI pectin methylesterase inhibitor protein, RGL rhamnogalacturonan lyase, XTH xyloglucan endotranglucosylase/hydrolase.
Figure 2
Figure 2
Model of floral stages at which abscission subfunctions may take place. Floral stage numbers are further defined elsewhere. Abscission signal receipt is expected to take place before anthesis because abscission regulators have accumulated by S12. These include the HAESA receptor kinase and multiple ethylene response factors. Signaling at or beyond anthesis is evidenced by accumulation at S13 of IDA ligand transcript required for abscission. Evidence that a subset of stamen AZ cells have completely separated by S15c is provided in Cai and Lashbrook. GH9 is Glycosyl Hydrolase Family 9; GH7 is Glycosyl Hydrolase Family 17.
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