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. 2010 May;76(9):3048-51.
doi: 10.1128/AEM.03127-09. Epub 2010 Mar 12.

Selection of bifidobacteria based on adhesion and anti-inflammatory capacity in vitro for amelioration of murine colitis

Julia Preising  1 , David Philippe, Marita Gleinser, Hua Wei, Stephanie Blum, Bernhard J Eikmanns, Jan-Hendrik Niess, Christian U Riedel
Affiliations

Selection of bifidobacteria based on adhesion and anti-inflammatory capacity in vitro for amelioration of murine colitis

Julia Preising et al. Appl Environ Microbiol. 2010 May.

Abstract

Adhesion and anti-inflammatory properties of eight strains of bifidobacteria were tested using the intestinal epithelial cell lines Caco-2, T84, and HT29. Two strains were selected for further assessment of their anti-inflammatory capacity in two murine models of colitis. In vivo results confirmed the high anti-inflammatory capacity of a Bifidobacterium bifidum strain.

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Figures

FIG. 1.
FIG. 1.
Adhesion (A, D, G) and inhibition of LPS-induced IL-8 secretion (B, E) or NF-κB reporter activity (H) of bifidobacteria tested in Caco-2 (A, B, C), T84 (D, E, F), and HT-29 clone 34 (G, H, I) cells. All of the values are the mean ± SEM of three independent experiments performed in triplicate. To identify strains with the most promising characteristics for in vivo application, adhesion was plotted against IL-8 (C, F) or NF-κB reporter activity (I). The bacterial strains used are B. bifidum S17, B. bifidum NCC189, B. lactis NCC362, B. adolescentis NCC251, B. bifidum S16, B. longum NCC2705, B. breve MB226, and B. longum/infantis E18. RLU, relative light units.
FIG. 2.
FIG. 2.
(A) Weight curves of Rag−/− mice that received a transfer of CD4+ CD45RBhigh T cells and were treated with B. bifidum S17 or B. longum/infantis E18 or control animals. At the end of the trial, the colons were removed from all of the animals and used to determine colonic weight/length ratios (C). n.s., no statistically significant difference. Representative colons (one animal of each experimental group) are shown (B). H&E-stained histological sections of the distal colon were scored for signs of inflammation (E). Representative histological sections (one animal of each experimental group) are shown in panel D. Statistical analysis was performed by analysis of variance with Bonferroni posttests for comparison to the colitic controls. All of the values are the mean ± SEM of six animals per group. Asterisks indicate a statistically significant difference between colitic controls and S17-treated animals (*, P < 0.05; **, P < 0.01; ***, P < 0.001). E18-treated animals and healthy controls were significantly different from colitic controls from day 10 until the end of the trial.
FIG. 3.
FIG. 3.
(A) Chemically induced weight loss of animals treated with B. bifidum S17 or control animals. (B) Inflammatory scores of H&E-stained histological sections of colonic biopsy samples from the animals shown in panel A at the end of the trial. Statistical analysis was performed by analysis of variance with Bonferroni posttests for comparison to the colitic controls. All of the values are the mean ± SEM of 9 or 10 animals per group.
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