Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10
- PMID: 20422209
- DOI: 10.1007/s00425-010-1170-5
Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10
Abstract
Skin color is an important factor in pear breeding programs. The degree of red coloration is determined by the content and composition of anthocyanins. In plants, many MYB transcriptional factors are involved in regulating anthocyanin biosynthesis. In this study, a R2R3-MYB transcription factor gene, PyMYB10, was isolated from Asian pear (Pyrus pyrifolia) cv. 'Aoguan'. Sequence analysis suggested that the PyMYB10 gene was an ortholog of MdMYB10 gene, which regulates anthocyanin biosynthesis in red fleshed apple (Malus x domestica) cv. 'Red Field'. PyMYB10 was identified at the genomic level and had three exons, with its upstream sequence containing core sequences of cis-acting regulatory elements involved in light responsiveness. Fruit bagging showed that light could induce expression of PyMYB10 and anthocyanin biosynthesis. Quantitative real-time PCR revealed that PyMYB10 was predominantly expressed in pear skins, buds, and young leaves, and the level of transcription in buds was higher than in skin and young leaves. In ripening fruits, the transcription of PyMYB10 in the skin was positively correlated with genes in the anthocyanin pathway and with anthocyanin biosynthesis. In addition, the transcription of PyMYB10 and genes of anthocyanin biosynthesis were more abundant in red-skinned pear cultivars compared to blushed cultivars. Transgenic Arabidopsis plants overexpressing PyMYB10 exhibited ectopic pigmentation in immature seeds. The study suggested that PyMYB10 plays a role in regulating anthocyanin biosynthesis and the overexpression of PyMYB10 was sufficient to induce anthocyanin accumulation.
Similar articles
-
PyMYB10 and PyMYB10.1 Interact with bHLH to Enhance Anthocyanin Accumulation in Pears.PLoS One. 2015 Nov 4;10(11):e0142112. doi: 10.1371/journal.pone.0142112. eCollection 2015. PLoS One. 2015. PMID: 26536358 Free PMC article.
-
The interaction of MYB, bHLH and WD40 transcription factors in red pear (Pyrus pyrifolia) peel.Plant Mol Biol. 2021 Jul;106(4-5):407-417. doi: 10.1007/s11103-021-01160-w. Epub 2021 Jun 11. Plant Mol Biol. 2021. PMID: 34117570
-
Persulfidation of transcription factor MYB10 inhibits anthocyanin synthesis in red-skinned pear.Plant Physiol. 2023 Jul 3;192(3):2185-2202. doi: 10.1093/plphys/kiad100. Plant Physiol. 2023. PMID: 36797801 Free PMC article.
-
Regulatory Mechanisms of Anthocyanin Biosynthesis in Apple and Pear.Int J Mol Sci. 2021 Aug 6;22(16):8441. doi: 10.3390/ijms22168441. Int J Mol Sci. 2021. PMID: 34445149 Free PMC article. Review.
-
Genome-wide identification of Pistacia R2R3-MYB gene family and function characterization of PcMYB113 during autumn leaf coloration in Pistacia chinensis.Int J Biol Macromol. 2021 Dec 1;192:16-27. doi: 10.1016/j.ijbiomac.2021.09.092. Epub 2021 Sep 20. Int J Biol Macromol. 2021. PMID: 34555399 Review.
Cited by
-
VvWRKY5 positively regulates wounding-induced anthocyanin accumulation in grape by interplaying with VvMYBA1 and promoting jasmonic acid biosynthesis.Hortic Res. 2024 Mar 25;11(5):uhae083. doi: 10.1093/hr/uhae083. eCollection 2024 May. Hortic Res. 2024. PMID: 38766531 Free PMC article.
-
Laser capture microdissection transcriptome (LCM RNA-seq) reveals BcDFR is a key gene in anthocyanin synthesis of non-heading Chinese cabbage.BMC Genomics. 2024 Apr 29;25(1):425. doi: 10.1186/s12864-024-10341-y. BMC Genomics. 2024. PMID: 38684983 Free PMC article.
-
GmMYB114 Facilitates the Synthesis of Anthocyanins in Soybean Sprouts under Blue Light.Plants (Basel). 2024 Apr 16;13(8):1107. doi: 10.3390/plants13081107. Plants (Basel). 2024. PMID: 38674516 Free PMC article.
-
The PpMYB75-PpDFR module reveals the difference between 'SR' and its bud variant 'RMHC' in peach red flesh.J Plant Res. 2024 Mar;137(2):241-254. doi: 10.1007/s10265-023-01512-1. Epub 2024 Jan 9. J Plant Res. 2024. PMID: 38194204
-
Multifaceted roles of WRKY transcription factors in abiotic stress and flavonoid biosynthesis.Front Plant Sci. 2023 Dec 15;14:1303667. doi: 10.3389/fpls.2023.1303667. eCollection 2023. Front Plant Sci. 2023. PMID: 38169626 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources