Ovine rotavirus strain LLR-85-based bovine rotavirus candidate vaccines: construction, characterization and immunogenicity evaluation
- PMID: 20488633
- PMCID: PMC7117303
- DOI: 10.1016/j.vetmic.2010.04.016
Ovine rotavirus strain LLR-85-based bovine rotavirus candidate vaccines: construction, characterization and immunogenicity evaluation
Abstract
Group A bovine rotaviruses (BRVs) are the most important cause of diarrheal diseases in neonatal calves and cause significant morbidity and mortality in the young animals, and epidemiologic surveillance of bovine rotavirus G genotypes conducted in various cattle populations throughout the world has shown that approximately 90% of the bovine rotavirus isolates belong to G6 and G10. Based on the modified Jennerian approach to immunization, we constructed and characterized a reassortant rotavirus stain, which bears a single bovine rotavirus VP7 gene encoding G genotype 6 specificity while the remaining 10 genes are derived from the ovine attenuated rotavirus LLR-85. The reassortant rotavirus strain, named as R191, and its parental virus strain LLR-85 were combined as bivalent vaccine candidates to inoculate the colostrums-deprived neonatal calves for evaluation of the immunogenicity. The calves were orally inoculated with the reassortant R191 (group 1), the parental rotavirus LLR-85 (group 2), or combined the R191 and LLR-85 (group 3), and serum specimens were detected to determine the immune response of IgG and IgA antibodies. Results showed that seroconversion to positivity for IgG and IgA antibodies occurred at postinoculation day (PID) 10 in all of the inoculated calves, and the highest titers of the serum IgG (range 1:800 to 1:6400) and IgA (range 1:800 to 1:3200) antibodies were obtained at PID 21 for all calves. Meanwhile, virus shedding was detected after inoculation, showing that the inoculated virus was positive in 2 of 77 fecal specimens (2.6%) collected from the inoculated calves during the first 7 days of oral inoculation with the rotavirus vaccine candidates. The results suggested that the rotavirus strains R191 and LLR-85 are promising bivalent vaccine candidates for the prevention of bovine G6 and G10 rotavirus infection.
Copyright © 2010 Elsevier B.V. All rights reserved.
Figures
Similar articles
-
The new pentavalent rotavirus vaccine composed of bovine (strain WC3) -human rotavirus reassortants.Pediatr Infect Dis J. 2006 Jul;25(7):577-83. doi: 10.1097/01.inf.0000220283.58039.b6. Pediatr Infect Dis J. 2006. PMID: 16804425 Review.
-
Determination of bovine rotavirus G genotypes in Kashmir, India.Rev Sci Tech. 2004 Dec;23(3):931-6. doi: 10.20506/rst.23.3.1538. Rev Sci Tech. 2004. PMID: 15861888 Review.
-
Modulation by colostrum-acquired maternal antibodies of systemic and mucosal antibody responses to rotavirus in calves experimentally challenged with bovine rotavirus.Vet Immunol Immunopathol. 2004 Jul;100(1-2):7-24. doi: 10.1016/j.vetimm.2004.02.007. Vet Immunol Immunopathol. 2004. PMID: 15182992 Free PMC article.
-
Construction and characterization of rhesus monkey rotavirus (MMU18006)- or bovine rotavirus (UK)-based serotype G5, G8, G9 or G10 single VP7 gene substitution reassortant candidate vaccines.Vaccine. 2003 Jun 20;21(21-22):3003-10. doi: 10.1016/s0264-410x(03)00120-8. Vaccine. 2003. PMID: 12798644
-
Prevalence of, and antigenic variation in, serotype G10 rotaviruses and detection of serotype G3 strains in diarrheic calves: implications for the origin of G10P11 or P11 type reassortant asymptomatic strains in newborn children in India.Arch Virol. 2002;147(1):143-65. doi: 10.1007/s705-002-8308-z. Arch Virol. 2002. PMID: 11855628
Cited by
-
Comparison of IgA Antibody Titer Induced by Human-Bovine Rotavirus Candidate Vaccine with Bovine Rotavirus and Rotarix.Arch Razi Inst. 2023 Feb 28;78(1):405-412. doi: 10.22092/ARI.2021.354821.1652. eCollection 2023 Feb. Arch Razi Inst. 2023. PMID: 37312718 Free PMC article.
-
Inhibition of rotavirus infectivity by a neoglycolipid receptor mimetic.Nutrients. 2011 Feb;3(2):228-44. doi: 10.3390/nu3020228. Epub 2011 Feb 17. Nutrients. 2011. PMID: 22254094 Free PMC article.
References
-
- Alfieri A.F., Alfieri A.A., Barreiros M.A., Leite J.P., Richtzenhain L.J. G and P genotypes of group A rotavirus strains circulating in calves in Brazil, 1996–1999. Vet. Microbiol. 2004;99:167–173. - PubMed
-
- Bai Z.S., Chen D.M., Shen S. Selection and characterization of strain LLR-85 for oral rotavirus live vaccine. Chin. J. Biol. 1994;7:49–51. (in Chinese)
-
- Bellinzoni R.C., Blackhall J., Terzolo H.R., Moreira A.R., Auza N., Mattion N., Micheo G.L., La Torre J.L., Scodeller E.A. Microbiology of diarrhoea in young beef and dairy calves in Argentina. Rev. Argent. Microbiol. 1990;22:130–136. - PubMed
-
- Bendali F., Bichet H., Schelcher F., Sanaa M. Pattern of diarrhoea in newborn beef calves in south-west France. Vet. Res. 1999;30:61–74. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous