Class-IIS restriction enzymes--a review
- PMID: 2055464
- DOI: 10.1016/0378-1119(91)90345-c
Class-IIS restriction enzymes--a review
Erratum in
- Gene 1991 Dec 20;109(1):169
Abstract
Class-IIS restriction enzymes (ENases-IIS) interact with two discrete sites on double-stranded DNA: the recognition site, which is 4-7 bp long, and the cleavage site, usually 1-20 bp away from the recognition site. The recognition sequences of ENases-IIS are totally (or partially) asymmetric and all of the characterized ENases-IIS are monomeric. A total of 35 ENases-IIS are described (80, if all isoschizomers are taken into consideration) together with ten related ENases (class IIT), and 15 cognate methyltransferases (MTases-IIS). The physical, chemical, and molecular properties of the ENases-IIS and MTases-IIS are reviewed and many unique applications of this class of enzymes are described, including: precise trimming of DNA; retrieval of cloned fragments; gene assembly; use as a universal restriction enzyme; cleavage of single-stranded DNA; detection of point mutations; tandem amplification; printing-amplification reaction; and localization of methylated bases.
Similar articles
-
The type IIB restriction endonucleases.Biochem Soc Trans. 2010 Apr;38(2):410-6. doi: 10.1042/BST0380410. Biochem Soc Trans. 2010. PMID: 20298193 Review.
-
Evolutionary relationship of Alw26I, Eco31I and Esp3I, restriction endonucleases that recognise overlapping sequences.Mol Genet Genomics. 2002 Jul;267(5):664-72. doi: 10.1007/s00438-002-0701-6. Epub 2002 Jun 19. Mol Genet Genomics. 2002. PMID: 12172806
-
Some class-IIS restriction endonucleases can cleave across a three-way DNA junction.Gene. 1994 Dec 2;150(1):63-6. doi: 10.1016/0378-1119(94)90858-3. Gene. 1994. PMID: 7959064
-
Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3).Gene. 1990 Aug 16;92(1-2):1-248. doi: 10.1016/0378-1119(90)90486-b. Gene. 1990. PMID: 2172084 Review.
-
Isolation and characterization of new restriction endonucleases from Vibrio parahaemolyticus: VpaK32I enzyme with the class-IIS heptanucleotide specificity, GCTCTTCN1/N4.Gene. 1992 Aug 1;117(1):103-6. doi: 10.1016/0378-1119(92)90496-c. Gene. 1992. PMID: 1644299
Cited by
-
Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues.Biotechnol Biofuels Bioprod. 2023 Oct 14;16(1):153. doi: 10.1186/s13068-023-02393-1. Biotechnol Biofuels Bioprod. 2023. PMID: 37838699 Free PMC article.
-
Standardizing cassette-based deep mutagenesis by Golden Gate assembly.Biotechnol Bioeng. 2024 Jan;121(1):281-290. doi: 10.1002/bit.28564. Epub 2023 Sep 26. Biotechnol Bioeng. 2024. PMID: 37750676
-
Golden Standard: a complete standard, portable, and interoperative MoClo tool for model and non-model proteobacteria.Nucleic Acids Res. 2023 Oct 27;51(19):e98. doi: 10.1093/nar/gkad758. Nucleic Acids Res. 2023. PMID: 37718823 Free PMC article.
-
An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly.Cell Rep Methods. 2023 Aug 22;3(8):100564. doi: 10.1016/j.crmeth.2023.100564. eCollection 2023 Aug 28. Cell Rep Methods. 2023. PMID: 37671021 Free PMC article.
-
Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI.Nucleic Acids Res. 2023 May 22;51(9):4467-4487. doi: 10.1093/nar/gkad228. Nucleic Acids Res. 2023. PMID: 36987874 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases