Use of plasmid profiles and restriction endonuclease digest in environmental studies of Listeria spp. from raw milk
- PMID: 2118791
- DOI: 10.1016/0168-1605(90)90006-q
Use of plasmid profiles and restriction endonuclease digest in environmental studies of Listeria spp. from raw milk
Abstract
Forty-eight isolates of Listeria spp. (19 L. monocytogenes, 27 L. innocua, 2 L. welshimeri) from bulk raw milk were screened for plasmid DNA. These isolates were collected over a period of 1 year. Only L. innocua harboured plasmids (8/27 strains) which ranged in size 10-44 megadaltons. The plasmid bearing strains could be arranged into three groups 10 Md, 44 Md and 44 + 10 Md. In two farms where Listeria innocua were persistent in bulk raw milk different plasmids profiles were found (farm 1 and 3). In species carrying similar plasmid profiles (44 Md) isolated from the same bulk tank raw milk samples over a period of 2-4 months (farm 2) no similarities in restriction endonuclease digest patterns of the plasmids were observed. This study suggests there may be a constant influx of Listeria spp. into raw milk supplies on the farm.
Similar articles
-
Prevalence and fingerprinting of Listeria monocytogenes strains isolated from raw whole milk in farm bulk tanks and in dairy plant receiving tanks.Appl Environ Microbiol. 2002 Jul;68(7):3366-70. doi: 10.1128/AEM.68.7.3366-3370.2002. Appl Environ Microbiol. 2002. PMID: 12089016 Free PMC article.
-
Prevalence of Listeria species in raw milk from farm bulk tanks in Nagano prefecture.J Vet Med Sci. 1998 Mar;60(3):311-4. doi: 10.1292/jvms.60.311. J Vet Med Sci. 1998. PMID: 9560778
-
Characterization of plasmids from Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.Int J Food Microbiol. 1998 Feb 17;39(3):231-6. doi: 10.1016/s0168-1605(97)00132-3. Int J Food Microbiol. 1998. PMID: 9553802
-
Antibiotic resistance in Listeria spp.Antimicrob Agents Chemother. 1999 Sep;43(9):2103-8. doi: 10.1128/AAC.43.9.2103. Antimicrob Agents Chemother. 1999. PMID: 10471548 Free PMC article. Review. No abstract available.
-
Incidence and significance of thermoduric bacteria in farm milk supplies: a reappraisal and review.J Appl Bacteriol. 1967 Aug;30(2):265-98. doi: 10.1111/j.1365-2672.1967.tb00299.x. J Appl Bacteriol. 1967. PMID: 4864318 Review. No abstract available.
Cited by
-
Comparative Analysis of Listeria monocytogenes Plasmids and Expression Levels of Plasmid-Encoded Genes during Growth under Salt and Acid Stress Conditions.Toxins (Basel). 2019 Jul 20;11(7):426. doi: 10.3390/toxins11070426. Toxins (Basel). 2019. PMID: 31330827 Free PMC article.
-
Comparative analysis of plasmids in the genus Listeria.PLoS One. 2010 Sep 2;5(9):e12511. doi: 10.1371/journal.pone.0012511. PLoS One. 2010. PMID: 20824078 Free PMC article.
-
Prevalence and fingerprinting of Listeria monocytogenes strains isolated from raw whole milk in farm bulk tanks and in dairy plant receiving tanks.Appl Environ Microbiol. 2002 Jul;68(7):3366-70. doi: 10.1128/AEM.68.7.3366-3370.2002. Appl Environ Microbiol. 2002. PMID: 12089016 Free PMC article.
-
Use of molecular typing methods to trace the dissemination of Listeria monocytogenes in a shrimp processing plant.Appl Environ Microbiol. 1996 Feb;62(2):705-11. doi: 10.1128/aem.62.2.705-711.1996. Appl Environ Microbiol. 1996. PMID: 8593073 Free PMC article.
-
Listeria monocytogenes, a food-borne pathogen.Microbiol Rev. 1991 Sep;55(3):476-511. doi: 10.1128/mr.55.3.476-511.1991. Microbiol Rev. 1991. PMID: 1943998 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous