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. 1990 Jan;10(1):43-50.
doi: 10.1016/0168-1605(90)90006-q.

Use of plasmid profiles and restriction endonuclease digest in environmental studies of Listeria spp. from raw milk

E Fistrovici  1 , D L Collins-Thompson
Affiliations

Use of plasmid profiles and restriction endonuclease digest in environmental studies of Listeria spp. from raw milk

E Fistrovici et al. Int J Food Microbiol. 1990 Jan.

Abstract

Forty-eight isolates of Listeria spp. (19 L. monocytogenes, 27 L. innocua, 2 L. welshimeri) from bulk raw milk were screened for plasmid DNA. These isolates were collected over a period of 1 year. Only L. innocua harboured plasmids (8/27 strains) which ranged in size 10-44 megadaltons. The plasmid bearing strains could be arranged into three groups 10 Md, 44 Md and 44 + 10 Md. In two farms where Listeria innocua were persistent in bulk raw milk different plasmids profiles were found (farm 1 and 3). In species carrying similar plasmid profiles (44 Md) isolated from the same bulk tank raw milk samples over a period of 2-4 months (farm 2) no similarities in restriction endonuclease digest patterns of the plasmids were observed. This study suggests there may be a constant influx of Listeria spp. into raw milk supplies on the farm.

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