Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990;114(3-4):175-89.
doi: 10.1007/BF01310747.

Initial events in bovine coronavirus infection: analysis through immunogold probes and lysosomotropic inhibitors

H R Payne  1 , J Storz, W G Henk
Affiliations

Initial events in bovine coronavirus infection: analysis through immunogold probes and lysosomotropic inhibitors

H R Payne et al. Arch Virol. 1990.

Abstract

The early events in the infection of human rectal tumor cells by bovine coronavirus were investigated by colloidal gold-mediated immunoelectron microscopy and by analysis of the effect of lysosomotropic weak bases on virus yield. Electron microscopic studies revealed sites of fusion between the virus envelope and the plasmalemma but fusion events along intracellular membranes were not observed despite extensive searches. Virion-antibody-colloidal gold complexes were, in fact, endocytosed by synchronously infected cells. These complexes were apparently non-infectious, and they accumulated in vacuoles that resembled secondary lysosomes. Exposure of cells to ammonium chloride or to methylamine during the first hour of infection had little inhibitory effect on the production of infectious virus. Chloroquine treatments were inhibitory but this effect depended on relatively late events in the infectious process. The chloroquine inhibitory step blocked infection of virus absorbed to cells that were exposed to buffers in the pH range of 4.4 to 8.4. These findings indicate that BCV penetrates its host cell by direct fusion with the plasmalemma and does not require an acidic intracellular compartment for infectious entry.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Andersen KB, Nexo BA. Entry of murine retrovirus into mouse fibroblasts. Virology. 1983;125:85–98. - PubMed
    1. Cassell S, Edwards J, Brown DT. Effects of lysosomotropic weak bases on infection of BHK-12 cells by Sindbis virus. J Virol. 1984;52:857–864. - PMC - PubMed
    1. Chasey D, Alexander DJ. Morphogenesis of avian infectious bronchitis virus in primary chick kidney cells. Arch Virol. 1976;52:101–111. - PMC - PubMed
    1. David-Ferreira JF, Manaker RA. An electron microscope study of the development of a mouse hepatitis virus in tissue culture cells. J Cell Biol. 1965;24:57–78. - PMC - PubMed
    1. Dimmock NJ. Initial stages in infection with animal virus. J Gen Virol. 1982;59:1–22. - PubMed

Publication types

LinkOut - more resources

-