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Comment
. 2011 Dec;131(12):2497-500.
doi: 10.1038/jid.2011.232. Epub 2011 Aug 11.

Deletion of PTENP1 pseudogene in human melanoma

Laura Poliseno, Adele Haimovic, Paul J Christos, Eleazar C Vega Y Saenz de Miera, Richard Shapiro, Anna Pavlick, Russell S Berman, Farbod Darvishian, Iman Osman
Comment

Deletion of PTENP1 pseudogene in human melanoma

Laura Poliseno et al. J Invest Dermatol. 2011 Dec.
No abstract available

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Figures

Figure 1
Figure 1. PTEN- and PTENP1-targeting microRNAs
a. Schematic representation of PTEN and PTENP1 3’UTR. The most upstream region of the 3’UTR (R1, dark grey) is highly conserved between PTEN and PTENP1. In the middle R2 region (light grey), the conservation drops dramatically. The downstream R3 region (white) is present only in PTEN (modified from Poliseno et al., 2010). b. List of the PTEN-targeting microRNA families validated so far. The region of the 3’UTR where the microRNA binding sites are located and the conservation of these sites in PTENP1 3’UTR are indicated. Out of a total of 18 PTEN-targeting microRNA families, 12 are PTENP1-targeting as well (grey). All these families have at least 1 binding site in the high homology R1 region.
Figure. 2
Figure. 2. Analysis of CDKN2A, CDKN2B, MTAP, PTENP1, PTEN, BRAF and NRAS status in human melanoma cell lines (a) and tissues (b)
a. 14 primary (WM series) and 14 metastatic (Sk-Mel series, 501Mel, A375 and 451Lu) melanoma cell lines and b. 23 primary (P1-P23) and 20 metastatic (M1-M20) melanoma tissues were analyzed by qPCR on genomic DNA to identify deletions at 9p21 (CDKN2A, CDKN2B and MTAP loci), 9p13 (PTENP1 locus) and 10q (PTEN locus). Mutations identified in exon 11 and 15 of BRAF and exon 2 and 3 of NRAS are reported. PTEN protein levels were analyzed in melanoma tissues by immunohistochemistry (IHC) and the percentage of positive cells is indicated. N/A: genomic qPCR not performed due to constraints on DNA availability. On the left, IHC shows lower PTEN levels in P5 tissue harboring partial PTEN and PTENP1 deletion compared to M5 tissue harboring partial PTEN deletion only.
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