Folding and oligomerization properties of a soluble and secreted form of the paramyxovirus hemagglutinin-neuraminidase glycoprotein
- PMID: 2219705
- DOI: 10.1016/0042-6822(90)90347-t
Folding and oligomerization properties of a soluble and secreted form of the paramyxovirus hemagglutinin-neuraminidase glycoprotein
Abstract
The paramyxovirus SV5 hemagglutinin-neuraminidase (HN) glycoprotein (a type II integral membrane protein) was converted into a soluble and secreted form (HN-F) by replacing the HN signal/anchor domain with a hydrophobic domain that can act as a cleavable signal sequence. Approximately 40% of the HN-F synthesized was secreted from cells (t1/2 approximately 2.5-3 hr). The extracellular HN-F molecules were identified as disulfide-linked dimers and the majority of the population of molecules were resistant to endoglycosidase H digestion. Examination of the oligomeric form of the secreted HN-F, by sucrose density gradient sedimentation, indicated that under conditions where HN was a tetramer, HN-F was found to be a dimer, and no extracellular HN-F monomeric species could be detected. Secreted HN-F was fully reactive with conformation-specific monoclonal antibodies and was enzymatically active as shown by HN-F having neuraminidase activity. Examination of the intracellular HN-F species indicated that HN-F monomers were slowly converted to the disulfide-linked form and that under the sucrose density gradient sedimentation conditions used the HN-F monomers aggregated. Some of the HN-F monomers were degraded intracellularly. These data are discussed in relationship to the seemingly different folding and oligomerization requirements for the intracellular transport of soluble and membrane bound forms of a glycoprotein. The soluble and biologically active form of HN may be suitable for further structural and enzymatic studies.
Similar articles
-
Structural requirements in the membrane-spanning domain of the paramyxovirus HN protein for the formation of a stable tetramer.Virology. 1995 Oct 20;213(1):263-70. doi: 10.1006/viro.1995.1569. Virology. 1995. PMID: 7483273
-
Intracellular maturation and transport of the SV5 type II glycoprotein hemagglutinin-neuraminidase: specific and transient association with GRP78-BiP in the endoplasmic reticulum and extensive internalization from the cell surface.J Cell Biol. 1989 Dec;109(6 Pt 2):3273-89. doi: 10.1083/jcb.109.6.3273. J Cell Biol. 1989. PMID: 2557352 Free PMC article.
-
Defective assembly and intracellular transport of mutant paramyxovirus hemagglutinin-neuraminidase proteins containing altered cytoplasmic domains.J Virol. 1990 Aug;64(8):3605-16. doi: 10.1128/JVI.64.8.3605-3616.1990. J Virol. 1990. PMID: 2164588 Free PMC article.
-
Flux of the paramyxovirus hemagglutinin-neuraminidase glycoprotein through the endoplasmic reticulum activates transcription of the GRP78-BiP gene.J Virol. 1991 Jul;65(7):3590-7. doi: 10.1128/JVI.65.7.3590-3597.1991. J Virol. 1991. PMID: 2041085 Free PMC article.
-
Homooligomerization of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 occurs before the acquisition of correct intramolecular disulfide bonds and mature immunoreactivity.J Virol. 1991 May;65(5):2362-71. doi: 10.1128/JVI.65.5.2362-2371.1991. J Virol. 1991. PMID: 1707981 Free PMC article.
Cited by
-
Producing Vaccines against Enveloped Viruses in Plants: Making the Impossible, Difficult.Vaccines (Basel). 2021 Jul 13;9(7):780. doi: 10.3390/vaccines9070780. Vaccines (Basel). 2021. PMID: 34358196 Free PMC article. Review.
-
Molecular recognition of human ephrinB2 cell surface receptor by an emergent African henipavirus.Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):E2156-65. doi: 10.1073/pnas.1501690112. Epub 2015 Mar 30. Proc Natl Acad Sci U S A. 2015. PMID: 25825759 Free PMC article.
-
Timing is everything: Fine-tuned molecular machines orchestrate paramyxovirus entry.Virology. 2015 May;479-480:518-31. doi: 10.1016/j.virol.2015.02.037. Epub 2015 Mar 12. Virology. 2015. PMID: 25771804 Free PMC article. Review.
-
Structure of the parainfluenza virus 5 (PIV5) hemagglutinin-neuraminidase (HN) ectodomain.PLoS Pathog. 2013;9(8):e1003534. doi: 10.1371/journal.ppat.1003534. Epub 2013 Aug 8. PLoS Pathog. 2013. PMID: 23950713 Free PMC article.
-
Paramyxovirus entry.Adv Exp Med Biol. 2013;790:95-127. doi: 10.1007/978-1-4614-7651-1_6. Adv Exp Med Biol. 2013. PMID: 23884588 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources