Color regulation in the archaebacterial phototaxis receptor phoborhodopsin (sensory rhodopsin II)
- PMID: 2252905
- DOI: 10.1021/bi00488a038
Color regulation in the archaebacterial phototaxis receptor phoborhodopsin (sensory rhodopsin II)
Abstract
Phoborhodopsin, a repellent phototaxis receptor in Halobacterium halobium, exhibits vibrational fine structure, a feature that has not been identified for any other rhodopsin pigment at physiological temperatures. This conclusion follows form analysis of the absorption properties of the pigment in H. halobium membranes containing native retinal and an array of retinal analogues. The absorption spectrum of the native pigment has a maximum at 487 nm with a pronounced shoulder at 460 nm; however, the bandwidth is that expected for a single retinylidene species. Gaussian band-shape simulation with a spacing corresponding to the vibrational frequencies of polyene stretching modes reproduces the structured absorption spectra of native pigment as well as of analogue phoborhodopsin. Absorption shifts produced by a series of dihydroretinal and other retinal analogues strongly indicate that the dominant factor regulating the color of the pigment is planarization of the retinal ring with respect to the polyene chain.
Similar articles
-
Photochemistry and photoinduced proton-transfer by pharaonis phoborhodopsin.Biochemistry (Mosc). 2001 Nov;66(11):1277-82. doi: 10.1023/a:1013187403599. Biochemistry (Mosc). 2001. PMID: 11743872 Review.
-
Molecular mechanism of photosignaling by archaeal sensory rhodopsins.Annu Rev Biophys Biomol Struct. 1997;26:223-58. doi: 10.1146/annurev.biophys.26.1.223. Annu Rev Biophys Biomol Struct. 1997. PMID: 9241419 Review.
-
Sensory rhodopsins I and II modulate a methylation/demethylation system in Halobacterium halobium phototaxis.Proc Natl Acad Sci U S A. 1989 Oct;86(20):7746-50. doi: 10.1073/pnas.86.20.7746. Proc Natl Acad Sci U S A. 1989. PMID: 2682623 Free PMC article.
-
All-trans/13-cis isomerization of retinal is required for phototaxis signaling by sensory rhodopsins in Halobacterium halobium.Biophys J. 1990 Apr;57(4):807-14. doi: 10.1016/S0006-3495(90)82600-X. Biophys J. 1990. PMID: 2344465 Free PMC article.
-
Photoreaction cycle of phoborhodopsin studied by low-temperature spectrophotometry.Biochemistry. 1991 Jul 30;30(30):7416-24. doi: 10.1021/bi00244a008. Biochemistry. 1991. PMID: 1830220
Cited by
-
Convergent evolution of animal and microbial rhodopsins.RSC Adv. 2023 Feb 13;13(8):5367-5381. doi: 10.1039/d2ra07073a. eCollection 2023 Feb 6. RSC Adv. 2023. PMID: 36793294 Free PMC article. Review.
-
Proton transfer pathway in anion channelrhodopsin-1.Elife. 2021 Dec 21;10:e72264. doi: 10.7554/eLife.72264. Elife. 2021. PMID: 34930528 Free PMC article.
-
Optical Switching Between Long-lived States of Opsin Transmembrane Voltage Sensors.Photochem Photobiol. 2021 Sep;97(5):1001-1015. doi: 10.1111/php.13428. Epub 2021 May 14. Photochem Photobiol. 2021. PMID: 33817800 Free PMC article.
-
Conversion of microbial rhodopsins: insights into functionally essential elements and rational protein engineering.Biophys Rev. 2017 Dec;9(6):861-876. doi: 10.1007/s12551-017-0335-x. Epub 2017 Nov 25. Biophys Rev. 2017. PMID: 29178082 Free PMC article. Review.
-
Microbial rhodopsins: wide distribution, rich diversity and great potential.Biophys Physicobiol. 2015 Dec 11;12:121-9. doi: 10.2142/biophysico.12.0_121. eCollection 2015. Biophys Physicobiol. 2015. PMID: 27493861 Free PMC article. Review.