Subchronic exposure to arsenic inhibits spermatogenesis and downregulates the expression of ddx3y in testis and epididymis of mice
- PMID: 22581830
- DOI: 10.1093/toxsci/kfs169
Subchronic exposure to arsenic inhibits spermatogenesis and downregulates the expression of ddx3y in testis and epididymis of mice
Abstract
Arsenic (As) is a ubiquitous environmental contaminant. Excess As exposure is considered one of the top health threats worldwide. As-induced Male reproductive toxicity is causing wide concern. The goal of this study is to determine whether subchronic As exposure inhibits Ddx3y expression, an Y-linked gene important in spermatogenesis and sperm maturation, and whether the inhibited expression of Ddx3y is closely associated with As-induced male reproductive toxicity Adult mice were given drinking water alone or water containing 1, 2, and 4mg/l arsenic trioxide (As(2)O(3)) for 60 days. After the treatment, the weights of testis and epididymis were analyzed. The sperm quality, spermatogenesis, and histological alteration of the testis and epididymis were observed by microscope. Furthermore, the expressions of Ddx3y gene and its protein in the testis and epididymis were examined by real-time reverse transcription PCR, Western blotting, and immunohistochemistry. Compared with untreated mice, the weights of testis and epididymis were reduced, sperm motility and the number of stage VII cells in the seminiferous epithelium section were decreased, sperm malformation ratio was increased, and histopathological alterations were observed in As-treated mice. The gene and protein expression of Ddx3y in testis and epididymis were significantly downregulated in As-exposed mice. Subchronic As exposure has detrimental effects on spermatogenesis and sperm development. It also downregulates Ddx3y expressions in testis and epididymis. Our results indicated that Ddx3y may be an important target gene of As and the downregulated expression of Ddx3y may be closely related to male reproductive toxicity induced by As.
Similar articles
-
Subchronic exposure to lead acetate inhibits spermatogenesis and downregulates the expression of Ddx3y in testis of mice.Reprod Toxicol. 2013 Dec;42:242-50. doi: 10.1016/j.reprotox.2013.10.003. Epub 2013 Oct 17. Reprod Toxicol. 2013. PMID: 24140753
-
Reduced testosterone and Ddx3y expression caused by long-term exposure to arsenic and its effect on spermatogenesis in mice.Environ Toxicol Pharmacol. 2018 Oct;63:84-91. doi: 10.1016/j.etap.2018.08.012. Epub 2018 Aug 18. Environ Toxicol Pharmacol. 2018. PMID: 30189373
-
NTP technical report on the toxicity studies of Dibutyl Phthalate (CAS No. 84-74-2) Administered in Feed to F344/N Rats and B6C3F1 Mice.Toxic Rep Ser. 1995 Apr;30:1-G5. Toxic Rep Ser. 1995. PMID: 12209194
-
Review on arsenic-induced toxicity in male reproductive system and its amelioration.Andrologia. 2017 Nov;49(9). doi: 10.1111/and.12791. Epub 2017 Jan 30. Andrologia. 2017. PMID: 28133775 Review.
-
Review on molecular and biochemical insights of arsenic-mediated male reproductive toxicity.Life Sci. 2018 Nov 1;212:37-58. doi: 10.1016/j.lfs.2018.09.045. Epub 2018 Sep 27. Life Sci. 2018. PMID: 30267786 Review.
Cited by
-
Casein and pea enriched high-protein diet attenuates arsenic provoked apoptosis in testicles of adult rats.Toxicol Res (Camb). 2023 Jun 14;12(4):551-563. doi: 10.1093/toxres/tfad043. eCollection 2023 Aug. Toxicol Res (Camb). 2023. PMID: 37663799 Free PMC article.
-
Transgenerational effects in DNA methylation, genotoxicity and reproductive phenotype by chronic arsenic exposure.Sci Rep. 2021 Apr 15;11(1):8276. doi: 10.1038/s41598-021-87677-y. Sci Rep. 2021. PMID: 33859283 Free PMC article.
-
Genome-wide analysis of DNA methylation in testis of male rat exposed to chlorpyrifos.Toxicol Res (Camb). 2020 Aug 4;9(4):509-518. doi: 10.1093/toxres/tfaa050. eCollection 2020 Jul. Toxicol Res (Camb). 2020. PMID: 32905263 Free PMC article.
-
Impact of environmental contaminants on reproductive health of male domestic ruminants: a review.Environ Sci Pollut Res Int. 2020 Feb;27(4):3819-3836. doi: 10.1007/s11356-019-06980-4. Epub 2019 Dec 16. Environ Sci Pollut Res Int. 2020. PMID: 31845245 Review.
-
Exposure to Concentrated Ambient PM2.5 Compromises Spermatogenesis in a Mouse Model: Role of Suppression of Hypothalamus-Pituitary-Gonads Axis.Toxicol Sci. 2018 Mar 1;162(1):318-326. doi: 10.1093/toxsci/kfx261. Toxicol Sci. 2018. PMID: 29165613 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials