Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects
- PMID: 22586215
- PMCID: PMC3459047
- DOI: 10.1113/jphysiol.2012.230185
Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects
Abstract
Skeletal muscle mitochondrial content varies extensively between human subjects. Biochemical measures of mitochondrial proteins, enzyme activities and lipids are often used as markers of mitochondrial content and muscle oxidative capacity (OXPHOS). The purpose of this study was to determine how closely associated these commonly used biochemical measures are to muscle mitochondrial content and OXPHOS. Sixteen young healthy male subjects were recruited for this study. Subjects completed a graded exercise test to determine maximal oxygen uptake (VO2peak) and muscle biopsies were obtained from the vastus lateralis. Mitochondrial content was determined using transmission electron microscopy imaging and OXPHOS was determined as the maximal coupled respiration in permeabilized fibres. Biomarkers of interest were citrate synthase (CS) activity, cardiolipin content, mitochondrial DNA content (mtDNA), complex I–V protein content, and complex I–IV activity. Spearman correlation coefficient tests and Lin's concordance tests were applied to assess the absolute and relative association between the markers and mitochondrial content or OXPHOS. Subjects had a large range of VO2peak (range 29.9–71.6ml min−1 kg−1) and mitochondrial content (4–15% of cell volume).Cardiolipin content showed the strongest association with mitochondrial content followed by CS and complex I activities. mtDNA was not related to mitochondrial content. Complex IV activity showed the strongest association with muscle oxidative capacity followed by complex II activity.We conclude that cardiolipin content, and CS and complex I activities are the biomarkers that exhibit the strongest association with mitochondrial content, while complex IV activity is strongly associated with OXPHOS capacity in human skeletal muscle.
Figures
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Comment in
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Skeletal muscle mitochondrial function: is it quality or quantity that makes the difference in insulin resistance?J Physiol. 2012 Dec 1;590(23):5935-6. doi: 10.1113/jphysiol.2012.241083. J Physiol. 2012. PMID: 23204102 Free PMC article. No abstract available.
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References
-
- Achten J, Gleeson M, Jeukendrup AE. Determination of the exercise intensity that elicits maximal fat oxidation. Med Sci Sports Exerc. 2002;34:92–97. - PubMed
-
- Andersen JL, Aagaard P. Myosin heavy chain IIX overshoot in human skeletal muscle. Muscle Nerve. 2000;23:1095–1104. - PubMed
-
- Anderson EJ, Boyle KE, Houmard JA, Neufer PD. Obesity is associated with reduced glutathione content, increased mitochondrial H2O2 emitting potential and a more oxidized redox environment in human skeletal muscle. Diabetes. 2008;57:A430–A430.
-
- Anderson EJ, Lustig ME, Boyle KE, Woodlief TL, Kane DA, Lin CT, Price JW, Kang L, Rabinovitch PS, Szeto HH, Houmard JA, Cortright RN, Wasserman DH, Neufer PD. Mitochondrial H2O2 emission and cellular redox state link excess fat intake to insulin resistance in both rodents and humans. J Clin Invest. 2009;119:573–581. - PMC - PubMed
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