Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12
- PMID: 2311584
- PMCID: PMC551742
- DOI: 10.1002/j.1460-2075.1990.tb08179.x
Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12
Abstract
The Myf-6 gene, a novel member of the human gene family of muscle determination factors has been detected by its highly conserved sequence coding for a putative helix-loop-helix domain. This sequence motif is a common feature of all Myf factors and other regulatory proteins. The new Myf gene is located on human chromosome 12, approximately 6.5 Kb upstream of the Myf-5 locus in a closely linked cluster of myogenic determination genes. Myf-6 cDNAs were isolated from human and mouse skeletal muscle, the only tissue in which expression of the corresponding mRNA was observed. In contrast to human primary muscle cell cultures which express moderate levels of Myf-6 mRNA, most established rodent muscle cell lines completely lack this mRNA. Myogenic 10T1/2 cells, however, induced by the expression of either pEMSV-Myf-4 or pEMSV-Myf-5 activate their endogenous mouse Myf-6 gene. Constitutive expression of Myf-6 cDNA in C3H 10T1/2 fibroblasts establishes the muscle phenotype at a similar frequency to the previously characterized myogenic factors. Moreover, muscle-specific CAT reporter constructs containing either the human myosin light chain (MLC) enhancer or the promoter of the embryonic myosin light chain gene are activated in NIH 3T3 fibroblasts or in CV1 kidney cells by cotransfection of Myf-6 expression vehicles. This transcriptional activation occurs in the absence of any apparent conversion of the cellular phenotype of the recipient cells. Glutathione-S-transferase fusion proteins with Myf-3, Myf-4 or Myf-5 specifically bind to a MEF-like consensus sequence present in the human MLC enhancer and the MLC1 emb promoter. In contrast, the Myf-6 hybrid protein interacts weakly with the same sequences showing lower affinity and reduced specificity. Since co-expressed pEMSV-Myf-6, nevertheless, is able to activate transcription of the MLC-CAT reporter constructs in non-muscle tissue culture cells, the different DNA binding properties in vitro might suggest that transactivation of gene expression by Myf-6 involves distinct binding sites and/or additional protein factors.
Similar articles
-
Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products.EMBO J. 1989 Dec 1;8(12):3617-25. doi: 10.1002/j.1460-2075.1989.tb08535.x. EMBO J. 1989. PMID: 2583111 Free PMC article.
-
A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.EMBO J. 1989 Mar;8(3):701-9. doi: 10.1002/j.1460-2075.1989.tb03429.x. EMBO J. 1989. PMID: 2721498 Free PMC article.
-
cAMP-dependent protein kinase represses myogenic differentiation and the activity of the muscle-specific helix-loop-helix transcription factors Myf-5 and MyoD.J Biol Chem. 1993 May 5;268(13):9869-78. J Biol Chem. 1993. PMID: 8387507
-
Co-operativity of functional domains in the muscle-specific transcription factor Myf-5.EMBO J. 1992 May;11(5):1843-55. doi: 10.1002/j.1460-2075.1992.tb05236.x. EMBO J. 1992. PMID: 1582413 Free PMC article.
-
Targeted inactivation of myogenic factor genes reveals their role during mouse myogenesis: a review.Int J Dev Biol. 1996 Feb;40(1):345-53. Int J Dev Biol. 1996. PMID: 8735947 Review.
Cited by
-
Genome-wide association study for growth traits in Blanco Orejinegro and Romosinuano cattle.Trop Anim Health Prod. 2023 Oct 17;55(6):358. doi: 10.1007/s11250-023-03743-9. Trop Anim Health Prod. 2023. PMID: 37848724 Free PMC article.
-
Epigenetic Control of Muscle Stem Cells: Focus on Histone Lysine Demethylases.Front Cell Dev Biol. 2022 May 20;10:917771. doi: 10.3389/fcell.2022.917771. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 35669509 Free PMC article. Review.
-
Selenium Deficiency Leads to Reduced Skeletal Muscle Cell Differentiation by Oxidative Stress in Mice.Biol Trace Elem Res. 2023 Apr;201(4):1878-1887. doi: 10.1007/s12011-022-03288-2. Epub 2022 May 16. Biol Trace Elem Res. 2023. PMID: 35576098
-
Maternal nutrition altered embryonic MYOD1, MYF5, and MYF6 gene expression in genetically fat and lean lines of chickens.Anim Biosci. 2022 Aug;35(8):1223-1234. doi: 10.5713/ab.21.0521. Epub 2022 Mar 1. Anim Biosci. 2022. PMID: 35240030 Free PMC article.
-
Homodimeric and Heterodimeric Interactions among Vertebrate Basic Helix-Loop-Helix Transcription Factors.Int J Mol Sci. 2021 Nov 28;22(23):12855. doi: 10.3390/ijms222312855. Int J Mol Sci. 2021. PMID: 34884664 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous