Buprenorphine and norbuprenorphine quantification in human plasma by simple protein precipitation and ultra-high performance liquid chromatography tandem mass spectrometry
- PMID: 23357637
- DOI: 10.1016/j.jpba.2012.12.023
Buprenorphine and norbuprenorphine quantification in human plasma by simple protein precipitation and ultra-high performance liquid chromatography tandem mass spectrometry
Abstract
A highly sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for the quantification of buprenorphine and its major metabolite norbuprenorphine in human plasma. In order to speed up the process and decrease costs, sample preparation was performed by simple protein precipitation with acetonitrile. To the best of our knowledge, this is the first application of this extraction technique for the quantification of buprenorphine in plasma. Matrix effects were strongly reduced and selectivity increased by using an efficient chromatographic separation on a sub-2 μm column (Acquity UPLC BEH C18 1.7 μm, 2.1×50 mm) in 5 min with a gradient of ammonium formate 20 mM pH 3.05 and acetonitrile as mobile phase at a flow rate of 0.4 ml/min. Detection was made using a tandem quadrupole mass spectrometer operating in positive electrospray ionization mode, using multiple reaction monitoring. The procedure was fully validated according to the latest Food and Drug Administration guidelines and the Société Française des Sciences et Techniques Pharmaceutiques. Very good results were obtained by using a stable isotope-labeled internal standard for each analyte, to compensate for the variability due to the extraction and ionization steps. The method was very sensitive with lower limits of quantification of 0.1 ng/ml for buprenorphine and 0.25 ng/ml for norbuprenorphine. The upper limit of quantification was 250 ng/ml for both drugs. Trueness (98.4-113.7%), repeatability (1.9-7.7%), intermediate precision (2.6-7.9%) and internal standard-normalized matrix effects (94-101%) were in accordance with international recommendations. The procedure was successfully used to quantify plasma samples from patients included in a clinical pharmacogenetic study and can be transferred for routine therapeutic drug monitoring in clinical laboratories without further development.
Copyright © 2012 Elsevier B.V. All rights reserved.
Similar articles
-
Urinary Buprenorphine, Norbuprenorphine and Naloxone Concentrations and Ratios: Review and Potential Clinical Implications.J Addict Med. 2020 Dec;14(6):e344-e349. doi: 10.1097/ADM.0000000000000676. J Addict Med. 2020. PMID: 32530884 Review.
-
Quantification of taxanes in biological matrices: a review of bioanalytical assays and recommendations for development of new assays.Bioanalysis. 2014 Apr;6(7):993-1010. doi: 10.4155/bio.14.48. Bioanalysis. 2014. PMID: 24806907 Review.
-
Hollow fiber liquid-phase microextraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry for the simultaneous determination of naloxone, buprenorphine and norbuprenorphine in human plasma.J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Mar 1;951-952:157-63. doi: 10.1016/j.jchromb.2014.01.029. Epub 2014 Jan 29. J Chromatogr B Analyt Technol Biomed Life Sci. 2014. PMID: 24566267
-
Fast quantification of ten psychotropic drugs and metabolites in human plasma by ultra-high performance liquid chromatography tandem mass spectrometry for therapeutic drug monitoring.J Chromatogr A. 2013 May 31;1292:160-72. doi: 10.1016/j.chroma.2012.12.071. Epub 2013 Jan 9. J Chromatogr A. 2013. PMID: 23380367
-
Quantification of nicotine, cotinine, trans-3'-hydroxycotinine and varenicline in human plasma by a sensitive and specific UPLC-tandem mass-spectrometry procedure for a clinical study on smoking cessation.J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3574-82. doi: 10.1016/j.jchromb.2011.09.046. Epub 2011 Oct 1. J Chromatogr B Analyt Technol Biomed Life Sci. 2011. PMID: 22014744
Cited by
-
High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Buprenorphine Evaluation in Plasma-Application to Pharmacokinetic Studies in Rabbits.Molecules. 2021 Jan 15;26(2):437. doi: 10.3390/molecules26020437. Molecules. 2021. PMID: 33467733 Free PMC article.
-
Simultaneous determination of four Sudan dyes in rat blood by UFLC-MS/MS and its application to a pharmacokinetic study in rats.J Pharm Anal. 2015 Aug;5(4):239-248. doi: 10.1016/j.jpha.2015.03.001. Epub 2015 Mar 18. J Pharm Anal. 2015. PMID: 29403937 Free PMC article.
-
High-sensitivity analysis of buprenorphine, norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide in plasma and urine by liquid chromatography-mass spectrometry.J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 15;939:23-31. doi: 10.1016/j.jchromb.2013.09.004. Epub 2013 Sep 8. J Chromatogr B Analyt Technol Biomed Life Sci. 2013. PMID: 24095872 Free PMC article.
-
Evaluation of therapeutics in three-dimensional cell culture systems by MALDI imaging mass spectrometry.Anal Chem. 2013 Jul 2;85(13):6295-302. doi: 10.1021/ac400519c. Epub 2013 Jun 11. Anal Chem. 2013. PMID: 23724927 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources