A familiar stranger: CD34 expression and putative functions in SVF cells of adipose tissue
- PMID: 23362435
- PMCID: PMC3557347
- DOI: 10.4252/wjsc.v5.i1.1
A familiar stranger: CD34 expression and putative functions in SVF cells of adipose tissue
Abstract
Human adipose tissue obtained by liposuction is easily accessible and an abundant potential source of autologous cells for regenerative medicine applications. After digestion of the tissue and removal of differentiated adipocytes, the so-called stromal vascular fraction (SVF) of adipose, a mix of various cell types, is obtained. SVF contains mesenchymal fibroblastic cells, able to adhere to culture plastic and to generate large colonies in vitro, that closely resemble bone marrow-derived colony forming units-fibroblastic, and whose expanded progeny, adipose mesenchymal stem/stromal cells (ASC), show strong similarities with bone marrow mesenchymal stem cells. The sialomucin CD34, which is well known as a hematopoietic stem cell marker, is also expressed by ASC in native adipose tissue but its expression is gradually lost upon standard ASC expansion in vitro. Surprisingly little is known about the functional role of CD34 in the biology and tissue forming capacity of SVF cells and ASC. The present editorial provides a short introduction to the CD34 family of sialomucins and reviews the data from the literature concerning expression and function of these proteins in SVF cells and their in vitro expanded progeny.
Keywords: CD34; Endothelial progenitors; Human adipose tissue; Mesenchymal stromal cells; Sialomucins.
Figures
![Figure 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3557347/bin/WJSC-5-1-g001.gif)
![Figure 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3557347/bin/WJSC-5-1-g002.gif)
Similar articles
-
Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media.Stem Cell Res Ther. 2018 May 11;9(1):130. doi: 10.1186/s13287-018-0886-1. Stem Cell Res Ther. 2018. PMID: 29751821 Free PMC article.
-
Serially Transplanted Nonpericytic CD146(-) Adipose Stromal/Stem Cells in Silk Bioscaffolds Regenerate Adipose Tissue In Vivo.Stem Cells. 2016 Apr;34(4):1097-111. doi: 10.1002/stem.2325. Epub 2016 Mar 9. Stem Cells. 2016. PMID: 26865460 Free PMC article.
-
The ratio of ADSCs to HSC-progenitors in adipose tissue derived SVF may provide the key to predict the outcome of stem-cell therapy.Clin Transl Med. 2018 Feb 7;7(1):5. doi: 10.1186/s40169-018-0183-8. Clin Transl Med. 2018. PMID: 29417261 Free PMC article.
-
Therapeutic Potential of Adipose Stem Cells.Adv Exp Med Biol. 2021;1341:15-25. doi: 10.1007/5584_2018_248. Adv Exp Med Biol. 2021. PMID: 30051318 Review.
-
Use of Freshly Isolated Human Adipose Stromal Cells for Clinical Applications.Aesthet Surg J. 2017 Jul 1;37(suppl_3):S4-S8. doi: 10.1093/asj/sjw270. Aesthet Surg J. 2017. PMID: 29025212 Review.
Cited by
-
Insights into the role of adipose-derived stem cells and secretome: potential biology and clinical applications in hypertrophic scarring.Stem Cell Res Ther. 2024 May 12;15(1):137. doi: 10.1186/s13287-024-03749-6. Stem Cell Res Ther. 2024. PMID: 38735979 Free PMC article. Review.
-
Inflammation and Starvation Affect Housekeeping Gene Stability in Adipose Mesenchymal Stromal Cells.Curr Issues Mol Biol. 2024 Jan 19;46(1):842-855. doi: 10.3390/cimb46010054. Curr Issues Mol Biol. 2024. PMID: 38275668 Free PMC article.
-
CD34-Structure, Functions and Relationship with Cancer Stem Cells.Medicina (Kaunas). 2023 May 12;59(5):938. doi: 10.3390/medicina59050938. Medicina (Kaunas). 2023. PMID: 37241170 Free PMC article. Review.
-
L-carnitine in a certain concentration increases expression of cell surface marker CD34 and apoptosis in the rat bone marrow CD34+ hematopoietic stem cells.Iran J Vet Res. 2021 Fall;22(4):264-271. doi: 10.22099/ijvr.2021.39045.5677. Iran J Vet Res. 2021. PMID: 35126533 Free PMC article.
-
Novel cell sources for bone regeneration.MedComm (2020). 2021 May 4;2(2):145-174. doi: 10.1002/mco2.51. eCollection 2021 Jun. MedComm (2020). 2021. PMID: 34766140 Free PMC article. Review.
References
-
- Scherberich A, Müller AM, Schäfer DJ, Banfi A, Martin I. Adipose tissue-derived progenitors for engineering osteogenic and vasculogenic grafts. J Cell Physiol. 2010;225:348–353. - PubMed
-
- Mizuno H. Adipose-derived stem and stromal cells for cell-based therapy: current status of preclinical studies and clinical trials. Curr Opin Mol Ther. 2010;12:442–449. - PubMed
-
- Güven S, Mehrkens A, Saxer F, Schaefer DJ, Martinetti R, Martin I, Scherberich A. Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue. Biomaterials. 2011;32:5801–5809. - PubMed
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous