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. 2013 Sep 5;501(7465):116-20.
doi: 10.1038/nature12423. Epub 2013 Aug 4.

Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

Giovanni D'Angelo  1 , Takefumi Uemura, Chia-Chen Chuang, Elena Polishchuk, Michele Santoro, Henna Ohvo-Rekilä, Takashi Sato, Giuseppe Di Tullio, Antonio Varriale, Sabato D'Auria, Tiziana Daniele, Fabrizio Capuani, Ludger Johannes, Peter Mattjus, Maria Monti, Piero Pucci, Roger L Williams, John E Burke, Frances M Platt, Akihiro Harada, Maria Antonietta De Matteis
Affiliations

Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

Giovanni D'Angelo et al. Nature. .

Abstract

Newly synthesized proteins and lipids are transported across the Golgi complex via different mechanisms whose respective roles are not completely clear. We previously identified a non-vesicular intra-Golgi transport pathway for glucosylceramide (GlcCer)--the common precursor of the different series of glycosphingolipids-that is operated by the cytosolic GlcCer-transfer protein FAPP2 (also known as PLEKHA8) (ref. 1). However, the molecular determinants of the FAPP2-mediated transfer of GlcCer from the cis-Golgi to the trans-Golgi network, as well as the physiological relevance of maintaining two parallel transport pathways of GlcCer--vesicular and non-vesicular--through the Golgi, remain poorly defined. Here, using mouse and cell models, we clarify the molecular mechanisms underlying the intra-Golgi vectorial transfer of GlcCer by FAPP2 and show that GlcCer is channelled by vesicular and non-vesicular transport to two topologically distinct glycosylation tracks in the Golgi cisternae and the trans-Golgi network, respectively. Our results indicate that the transport modality across the Golgi complex is a key determinant for the glycosylation pattern of a cargo and establish a new paradigm for the branching of the glycosphingolipid synthetic pathway.

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