Wild-type and innate immune-deficient mice are not susceptible to the Middle East respiratory syndrome coronavirus
- PMID: 24197535
- PMCID: PMC3917065
- DOI: 10.1099/vir.0.060640-0
Wild-type and innate immune-deficient mice are not susceptible to the Middle East respiratory syndrome coronavirus
Abstract
The Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerging highly pathogenic virus causing almost 50 % lethality in infected individuals. The development of a small-animal model is critical for the understanding of this virus and to aid in development of countermeasures against MERS-CoV. We found that BALB/c, 129/SvEv and 129/SvEv STAT1 knockout mice are not permissive to MERS-CoV infection. The lack of infection may be due to the low level of mRNA and protein for the MERS-CoV receptor, dipeptidyl peptidase 4 (DPP4), in the lungs of mice. The low level of DPP4 in the lungs likely contributes to the lack of viral replication in these mouse models and suggests that a transgenic mouse model expressing DPP4 to higher levels is necessary to create a mouse model for MERS-CoV.
Figures
Similar articles
-
Acute Respiratory Infection in Human Dipeptidyl Peptidase 4-Transgenic Mice Infected with Middle East Respiratory Syndrome Coronavirus.J Virol. 2019 Mar 5;93(6):e01818-18. doi: 10.1128/JVI.01818-18. Print 2019 Mar 15. J Virol. 2019. PMID: 30626685 Free PMC article.
-
CD8+ T Cells and Macrophages Regulate Pathogenesis in a Mouse Model of Middle East Respiratory Syndrome.J Virol. 2016 Dec 16;91(1):e01825-16. doi: 10.1128/JVI.01825-16. Print 2017 Jan 1. J Virol. 2016. PMID: 27795435 Free PMC article.
-
A Comparative Review of Animal Models of Middle East Respiratory Syndrome Coronavirus Infection.Vet Pathol. 2016 May;53(3):521-31. doi: 10.1177/0300985815620845. Epub 2016 Feb 11. Vet Pathol. 2016. PMID: 26869154 Review.
-
Animal models of Middle East respiratory syndrome coronavirus infection.Antiviral Res. 2015 Oct;122:28-38. doi: 10.1016/j.antiviral.2015.07.005. Epub 2015 Jul 17. Antiviral Res. 2015. PMID: 26192750 Free PMC article. Review.
-
Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease.J Virol. 2015 Apr;89(7):3659-70. doi: 10.1128/JVI.03427-14. Epub 2015 Jan 14. J Virol. 2015. PMID: 25589660 Free PMC article.
Cited by
-
Characterization of a mouse-adapted strain of bat severe acute respiratory syndrome-related coronavirus.J Virol. 2023 Sep 28;97(9):e0079023. doi: 10.1128/jvi.00790-23. Epub 2023 Aug 21. J Virol. 2023. PMID: 37607058 Free PMC article.
-
Cell and Animal Models for SARS-CoV-2 Research.Viruses. 2022 Jul 9;14(7):1507. doi: 10.3390/v14071507. Viruses. 2022. PMID: 35891487 Free PMC article. Review.
-
Comparison of Experimental Middle East Respiratory Syndrome Coronavirus Infection Acquired by Three Individual Routes of Infection in the Common Marmoset.J Virol. 2022 Feb 23;96(4):e0173921. doi: 10.1128/JVI.01739-21. Epub 2021 Dec 15. J Virol. 2022. PMID: 34908447 Free PMC article.
-
Neurological manifestations of coronavirus infections, before and after COVID-19: a review of animal studies.J Neurovirol. 2021 Dec;27(6):864-884. doi: 10.1007/s13365-021-01014-7. Epub 2021 Nov 2. J Neurovirol. 2021. PMID: 34727365 Free PMC article. Review.
-
Mouse Models for the Study of SARS-CoV-2 Infection.Comp Med. 2021 Oct 1;71(5):383-397. doi: 10.30802/AALAS-CM-21-000031. Epub 2021 Oct 5. Comp Med. 2021. PMID: 34610856 Free PMC article. Review.
References
-
- Chan J. F., Chan K. H., Choi G. K., To K. K., Tse H., Cai J. P., Yeung M. L., Cheng V. C., Chen H. & other authors (2013). Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation. J Infect Dis 207, 1743–1752 10.1093/infdis/jit123 - DOI - PMC - PubMed
-
- Corman V. M., Eckerle I., Bleicker T., Zaki A., Landt O., Eschbach-Bludau M., van Boheemen S., Gopal R., Ballhause M. & other authors (2012a). Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Eurosurveillance 17, 39. - PubMed
-
- Corman V. M., Muller M. A., Costabel U., Timm J., Binger T., Meyer B., Kreher P., Lattwein E., Eschbach-Bludau M. & other authors (2012b). Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Eurosurveillance 17, 49 - PubMed
-
- de Wit E., Prescott J., Baseler L., Bushmaker T., Thomas T., Lackemeyer M. G., Martellaro C., Milne-Price S., Haddock E. & other authors (2013). The Middle East respiratory syndrome coronavirus (MERS-CoV) does not replicate in Syrian hamsters. PLoS ONE 8, e69127 10.1371/journal.pone.0069127 - DOI - PMC - PubMed
-
- Drosten C., Seilmaier M., Corman V. M., Hartmann W., Scheible G., Sack S., Guggemos W., Kallies R., Muth D. & other authors (2013). Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection. Lancet Infect Dis 13, 745–751 10.1016/S1473-3099(13)70154-3 - DOI - PMC - PubMed
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous