Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes
- PMID: 2429313
- PMCID: PMC386778
- DOI: 10.1073/pnas.83.20.7648
Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes
Abstract
A computer analysis of the amino acid sequences from the putative gene products of retroviral pol genes has revealed a 150-residue segment that is homologous with the ribonuclease H of Escherichia coli. The segment occurs at the carboxyl terminus of the region assigned to the 90-kDa reverse transcriptase polypeptide. In contrast, a section nearer the amino terminus of this sequence can be aligned with nonretroviral polymerases. The order of activities in the pol gene is thus: polymerase-ribonuclease-endonuclease. On another note, all retroviral endonuclease sequences contain a consensus zinc-binding "finger." This should not be confused with the well-known zinc requirement of reverse transcriptases.
Similar articles
-
Ribosome-inhibiting proteins, retroviral reverse transcriptases, and RNase H share common structural elements.Proteins. 1988;3(1):53-9. doi: 10.1002/prot.340030105. Proteins. 1988. PMID: 2453878
-
Analysis of the primary structure of the long terminal repeat and the gag and pol genes of the human spumaretrovirus.J Virol. 1988 May;62(5):1590-7. doi: 10.1128/JVI.62.5.1590-1597.1988. J Virol. 1988. PMID: 2451755 Free PMC article.
-
Proteolytic processing of avian sarcoma and leukosis viruses pol-endo recombinant proteins reveals another pol gene domain.J Virol. 1987 Feb;61(2):534-42. doi: 10.1128/JVI.61.2.534-542.1987. J Virol. 1987. PMID: 2433465 Free PMC article.
-
Ribonucleases H of retroviral and cellular origin.Pharmacol Ther. 1990;48(2):259-80. doi: 10.1016/0163-7258(90)90083-e. Pharmacol Ther. 1990. PMID: 1963496 Review.
-
Structural and antigenic characterization of the proteins of human T-cell leukemia viruses and their relationships to the gene products of other retroviruses.Princess Takamatsu Symp. 1984;15:147-57. Princess Takamatsu Symp. 1984. PMID: 6100635 Review.
Cited by
-
Retroviral RNase H: Structure, mechanism, and inhibition.Enzymes. 2021;50:227-247. doi: 10.1016/bs.enz.2021.07.007. Epub 2021 Sep 24. Enzymes. 2021. PMID: 34861939 Free PMC article. Review.
-
Avoiding Drug Resistance in HIV Reverse Transcriptase.Chem Rev. 2021 Mar 24;121(6):3271-3296. doi: 10.1021/acs.chemrev.0c00967. Epub 2021 Jan 28. Chem Rev. 2021. PMID: 33507067 Free PMC article. Review.
-
The Old and the New: Prospects for Non-Integrating Lentiviral Vector Technology.Viruses. 2020 Sep 29;12(10):1103. doi: 10.3390/v12101103. Viruses. 2020. PMID: 33003492 Free PMC article. Review.
-
Discovery and Development of Anti-HIV Therapeutic Agents: Progress Towards Improved HIV Medication.Curr Top Med Chem. 2019;19(18):1621-1649. doi: 10.2174/1568026619666190712204603. Curr Top Med Chem. 2019. PMID: 31424371 Free PMC article. Review.
-
X-ray crystal structure of the N-terminal region of Moloney murine leukemia virus integrase and its implications for viral DNA recognition.Proteins. 2017 Apr;85(4):647-656. doi: 10.1002/prot.25245. Epub 2017 Feb 3. Proteins. 2017. PMID: 28066922 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources