Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Feb 6;53(3):393-406.
doi: 10.1016/j.molcel.2014.01.009.

Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli

Katsutoshi Imamura  1 , Naoto Imamachi  2 , Gen Akizuki  2 , Michiko Kumakura  3 , Atsushi Kawaguchi  3 , Kyosuke Nagata  3 , Akihisa Kato  4 , Yasushi Kawaguchi  4 , Hiroki Sato  5 , Misako Yoneda  5 , Chieko Kai  5 , Tetsushi Yada  6 , Yutaka Suzuki  7 , Toshimichi Yamada  8 , Takeaki Ozawa  8 , Kiyomi Kaneki  9 , Tsuyoshi Inoue  9 , Mika Kobayashi  9 , Tatsuhiko Kodama  9 , Youichiro Wada  10 , Kazuhisa Sekimizu  1 , Nobuyoshi Akimitsu  11
Affiliations
Free article

Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli

Katsutoshi Imamura et al. Mol Cell. .
Free article

Erratum in

Abstract

Although thousands of long noncoding RNAs (lncRNAs) are localized in the nucleus, only a few dozen have been functionally characterized. Here we show that nuclear enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, is induced by influenza virus and herpes simplex virus infection as well as by Toll-like receptor3-p38 pathway-triggered poly I:C stimulation, resulting in excess formation of paraspeckles. We found that NEAT1 facilitates the expression of antiviral genes including cytokines such as interleukin-8 (IL8). We found that splicing factor proline/glutamine-rich (SFPQ), a NEAT1-binding paraspeckle protein, is a repressor of IL8 transcription, and that NEAT1 induction relocates SFPQ from the IL8 promoter to the paraspeckles, leading to transcriptional activation of IL8. Together, our data show that NEAT1 plays an important role in the innate immune response through the transcriptional regulation of antiviral genes by the stimulus-responsive cooperative action of NEAT1 and SFPQ.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources

-